FastQCFastQC Report
Wed 8 May 2024
EGAF00007966611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007966611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539303
Total Bases23.7 Mbp
Sequences flagged as poor quality0
Sequence length44
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACTGAGCCTTCACTGGTTCAGCTCCCTCGGTCGAGTACGCT32310.5991066246618321No Hit
GGCGCCCGCGGTTCAACCAATGGTAACACAAGAACTGAGCCTTC30920.5733326163585221No Hit
ACCCTGACCACGCGGACCGGTGGAAGGACCTTCGCCCGGATCAT26780.4965668650090951No Hit
CGAGATGTTTCCATCCAGAGGTCTCCGCAGCGGAAAAAATTGGC25500.47283252642762974No Hit
GGCCAGTTCGGAAGCGGACAGGTCGCTTTTCGGGATATAGTGAT21120.39161658659417803No Hit
ATACGGATAAGAACTACGGGCAGTGGCTGTAACCCTCTTAACCG18140.33636007958420405No Hit
GTCGCTTGGACGAGTCGGCAGGGTTGCGCCGGTTTCGTTGGCGG16650.30873182607921706No Hit
TTCCTGGTGATATTCAAAGTAGTCTGCTTCACCGACCGGGTGGA15670.2905602231027827No Hit
CGGACCTTCGCCCGGGTCATCTGCCGGGCCCTGTTCGATGGCAC15140.28073272353389467No Hit
AACCCTCTTAACCGCTGTATCGGCGAGAAGCTCTTCTGAGGGGC14800.27442828984819295No Hit
CATATCCGGTTCACCGTCCGGGCCACCTTCCTGGTGATATTCGA14150.2623756960372926No Hit
CGGTTCGCCGTCCGGGCCACCTTCCTGGTGATATTCAAAGTAAT13610.25236277194823686No Hit
CGGACGCGTTGGCAGGGTCGCGCCAGTTTCGTTTGCAGTCAGAG13540.251064800307063No Hit
GCCCTGACCGCGCGGGCCGGTGGACGGACCCTCACCCGGGTCGT13330.24717088538354134No Hit
TTCCTGGTGGTATTCGAAATAGTCTGCTTCGCCGACCGGGTGGA13290.24642918730287058No Hit
GGTGGATGGGCCTTCACCAGGATCGTCAGCCGGGCCCTGCTCGA11660.2162049905155358No Hit
GTCAGACGGACGAGTTGGCAGGGTTGCGCCGGTTTCGTTTGCGG10080.18690791632903953No Hit
CGGACGAGTCGGCAGGGTAGCACCGGTTTCGTTCGCGGTCAGTG9870.18301400140551785No Hit
ACCAGTAGCAACTACCGCCAGATCGCGGTTCGGGTACGGTGCAA9100.1687363133526051No Hit
TGCCGCCAGTTCAGATGCGCTCAGATCGGATTTCGGGATATAGT9040.16762376623159894No Hit
CGGTTCGCCATCCGGACCGCCTTCCTGGTGATACTCGAAGTAGT8500.15761084214254323No Hit
ACCCTGACCGCGCGGGCCGGTGGACGGGCCTTCGCCCGGGTCAT8270.1533460781786862No Hit
GGCATCGGTGAAGCCAGTGCCGAATGCGTCACCCAGACCTTTGA8220.1524189555778477No Hit
GCAACCGGTGCGTTCTGCCAGGGACGGGTCCAGGTCATCAGACA7820.1450019747711398No Hit
AGGCGGGATGGCGATGAAGGACGCGTGCGGGCGCGGACGACGCG7680.14240603148879202No Hit
AGTTTCGCTACCGTTGAAGTGCATGTAGGTGGTGCGGGTTTCCA7320.1357307487627549No Hit
GATTTCCGGGGTTTTGACAGAACGCAGCAGGTCAGCGATGGCCG7220.13387650356107791No Hit
CTGACCACGGTGTTTACCGAACCAACCACCTTTTTTGCGACGGC7200.1335056545207425No Hit
TTTCTGCAGGATGTTCTGTACCGCGTCGGACGGGGTTTTGGCAC7090.13146598479889784No Hit
CAGCGTCGGAATGGAGCGACGGGTCTTGATACCGTTGCCGCTTT6750.12516155111319613No Hit
ATCGCCCGGAACTACCTGGCTAGTGTGACCGGTCTCGGCAGCGG6580.12200933427034524No Hit
GATGGTGGATTCGCTACGGGAACGGCGCTGCACAACGTGACGGG6240.11570490058464351No Hit
AGAGGACGGACGGCTACCCTGTTTGCCGTTCCAGTAGGCCTGTG6090.11292353278212805No Hit
TGACCTATTTAAATACGAGCCTCAGTCAGACGGACGAGTCGGCA5980.11088386306028336No Hit
GCGAGTCTGATCGGTGATAACATAACGAGTTTCGATCATATCTT5880.10902961785860639No Hit
ACCCTGAACTGGGGTAGAGGTTACAGTTTGCGCACGAGCAGAGG5570.10328145773340774No Hit
GCTCAGCTGAGACGGCTGCATGTTTTTGCCGGCCAGGTGTGCTT5540.10272518417290466No Hit
TTACGCGTTGACAATACCGTAGGTCGTCAGCGAGATGTTTCCAT5460.10124178801156308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)