FastQCFastQC Report
Wed 8 May 2024
EGAF00007966612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007966612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539303
Total Bases16.7 Mbp
Sequences flagged as poor quality0
Sequence length31
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCGCCACACCCAGAAAGCACCGAAACGTA70071.2992696128150594No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGTA36360.6742035553297496No Hit
CGTCGTCATACCCAGAAAGCACCGAAACGCA34020.6308142176105084No Hit
GGCCGTCGCCCGTTCTTCCACCCGGTAGGTG27750.5145530434653618No Hit
GCTGTTGCAGCGGCGCGTGCACAGGGCCGCT21540.3994044164412213No Hit
TCTCCACCGCGTCGCCCGCCGCCGGGTCGTC21040.39013319043283645No Hit
CAGACCGATGCCCTGACCGAAGGTCTGTCTG19040.35304828639929686No Hit
GACGCACTGACGGAAGGTCTGTCCGATGAAC18160.33673092862453946No Hit
GAGCGTGCCCGTGGCCGCGGCCGCGGTCGCG17340.3215261179707882No Hit
CAGACCGACGCCCTGACTGAAGGTCTGTCCG16430.3046524866355277No Hit
GGCCGTCGTCCTTTTTTCCACCCGGTTGGCG15510.28759343078009947No Hit
CGTGGTGAAAAACGTCCGCGTTCCCCGTCCA15350.2846266384574163No Hit
GGTAGCCGTGAACGCGCACGTGGTCGTGGTC14410.2671967335616527No Hit
GAGCGTGCCCGTGGCCGTGGTCGCGGCCGCG13520.2506939512667276No Hit
CCGCGTCGCCCGCCGCCGGGCCGTCGTCCGT12690.2353037160928087No Hit
GACGCCCTGACGGAGGGTCTGAGCGATGAAC11180.2073046135474863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9650.1789346619618285No Hit
GGCCGTCGTCCGTTCTTCCACCCGGTTGGTG9590.17782211484082233No Hit
AACTACCCGCGTGATTCTAAAACCATTATCA9550.17708041676015154No Hit
GTAGCTGTTGCTCGCTCCCAGGGTCGTTACA9030.16743834171143124No Hit
GGTTCCCGTGAACGCGCACGTGGTCGTGGCC8840.16391527582824497No Hit
AGCGGTGACTGTGGCGGCATCCTGCGCTGCC8410.15594202146103397No Hit
CCGGTAGGCGAGGCGGACTATTTCGAATACC8390.15557117242069857No Hit
CAGGGTGCTCTGACTCTGTCTCTGCCGAAAC8080.14982301229549994No Hit
AAGCTGAAGGAAGCGGAAGCACGTGCCGAGT7940.14722706901315216No Hit
ACCCTGTCCCTGTCTCAGCTGTACTCCCACA7820.1450019747711398No Hit
GAGAAAACGAAACAGACTCTGGCTTCTGTTT7640.14166433340812123No Hit
ATTGCTGCCGTTGGCTCTCCAGTGAAGTCTA7520.13943923916610884No Hit
GTGTCCCCGAACCGTCAATTCATTCCATACC7450.13814126752493494No Hit
ATTGAACAGAAACAGCTGCTGCAGGGTGACG7340.13610159780309028No Hit
CAGGACAGCCTGCCGGATACCAAAGCATCTG6690.12404900399218992No Hit
ATGGGTGCAGACATGGTAGAACTGGACATTC6660.12349273043168682No Hit
ACCCAGAACCCAGTGGAAAACTACATCGACT6280.1164465986653143No Hit
CAGGGCCGTTATACTACCAACGACGGTTACG6190.11477777798380502No Hit
GCGCAGGGTCGCTACACTACCGACGACGGCT5940.11014216497961257No Hit
ACCGCGTCCGGTGAAGAAGTAGCAGTTCTGA5890.10921504237877408No Hit
CAGACCCCGGAACAGAGCACCCCGTCCCGCA5670.10513570293508472No Hit
CCGGTAGGCGAAGCAGACTACTTCGAGTATC5540.10272518417290466No Hit
CAGAAACAGCTGCTGCAGGGTGATGTTGAAG5420.10050008993089227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)