FastQCFastQC Report
Wed 31 May 2023
EGAF00007967470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007967470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134840606
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT8313280.6165264490134374No Hit
CCCGTTACATCTTCCGCGCAGGACGACTCGATCAGTGAGCTATTACGCTT7185130.5328609988596461No Hit
CCCCGTTACATCTTCCGCGCAGGACGACTCGATCAGTGAGCTATTACGCT5353130.3969968808950622No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG4912300.36430420670165187No Hit
CCTCACGGTACTGGTTCACTATCGGTCGATCACGAGTATTTAGCCTTGGA4178060.3098517667593395No Hit
GTTGATTTCTGTTCCTGCAGCTACTTAGATGTTTCAGTTCACTGCGTTCG4162280.3086814961362603No Hit
CCCCCATCGCATCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCA4156370.30824320086487894No Hit
CTCTGACACACTCTAGCCGTGCAGTCACCAATGCAATTCCCAGGTTAAGC3988960.2958278013078642No Hit
CCCCATTCGGACATCTGCGGATCAAAGCTTGTTTGCCAGCTCCCCGCAGC3807590.2823771053060975No Hit
CCCACTTCGTTTCCCACTTAGCCATATTTAGGGACCTTAGCTGGCGGTCT3800380.2818423999073395No Hit
GCCATGGATAGATCACTTGGTTTCGGGTCTACACCCAGCGACTGAACGCC3777020.2801099840800182No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC3541740.26266123425757965No Hit
CACCATTTTATTGCAACCCCTTCATCCTTCTGGCGCGAGCCAGTCAAACT3350410.24847188835683517No Hit
CCCCACTTTCACCCTCAGGTCGTATGCGGTATTAGCTAATCTTTCGACTA3337970.24754931759947743No Hit
CTCGGTTGATTTCTGTTCCTGCAGCTACTTAGATGTTTCAGTTCACTGCG3291950.24413639909034524No Hit
GCCTTTCACCCCTATCCACAGCTCATCCCCTAACTTTTCAACGTTAGTGG3272420.24268802233060272No Hit
GTTACATCTTCCGCGCAGGACGACTCGATCAGTGAGCTATTACGCTTTCT3246970.24080060868311431No Hit
CCCCATCGCATCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAG3156020.23405560784857346No Hit
GCACCATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTT3147610.2334319084860832No Hit
ACTGAATGTAAGTCGCTGACCCATTATACAAAAGGTACGCCGTCACCCGT2975060.2206353181177486No Hit
GTCCCGCCCTACTTGTCGTACACCTAGTTCCACAATACTGTTTTCGCATA2921370.2166535798570944No Hit
CCCCAGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA2902420.21524821684648912No Hit
CCCGTCAATTCCTTTGAGTTTTAATCTTGCGACCGTACTCCCCAGGCGGT2814610.20873608355038098No Hit
CCCACTTTCACCCTCAGGTCGTATGCGGTATTAGCTAATCTTTCGACTAG2791490.20702146651580608No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATCCTTTACAAGACACCTTC2732550.2026503796638232No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTCGGTCTCGCAGTTAAGCACGCT2705950.2006776801344248No Hit
CCCCAACCATGACAGCGCTCTACCCCCGAAGGTGAGACACGAGGCACTAC2638230.19565545411446755No Hit
CCCGAGTTCTCTCAAGCGCCTTAGAATACTCATCTCGCCCACCTGTGTCG2601130.19290405740241184No Hit
CTTGGTTTCGGGTCTACACCCAGCGACTGAACGCCCTATTCGGACTCGCT2599020.19274757634951595No Hit
GCCACTGAATGTAAGTCGCTGACCCATTATACAAAAGGTACGCCGTCACC2500650.18545229617256392No Hit
CCGTTACATCTTCCGCGCAGGACGACTCGATCAGTGAGCTATTACGCTTT2436460.18069186072925242No Hit
GTCGATCACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATCTTCAGACA2281360.1691893909168578No Hit
GCGGTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTC2263850.16789082066273123No Hit
CTCCTGACTGTTTTAGCCTTCCCACTTCGTTTCCCACTTAGCCATATTTA2263140.16783816590085632No Hit
GGTCGATCACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATCTTCAGAC2163260.16043090165287452No Hit
CCCGAAGTTACGGTACCAATTTGCCGAGTTCCTTCTCCCGAGTTCTCTCA2075020.15388687885309563No Hit
CCTCCCTCTGACACACTCTAGCCGTGCAGTCACCAATGCAATTCCCAGGT2052860.15224345698950656No Hit
CTCCTACCATGCACTTACGTGCATCCGCAGCTTCGGTATATGGCTTAGCC1971360.14619928361935722No Hit
ATCGAATTAATCCACATCATCCACCGCTTGTGCGGGTCCCCGTCAATTCC1952520.14480207838876075No Hit
CACCATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTTG1950870.14467971168862886No Hit
CCCACTTAGCCATATTTAGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCC1936040.143579894620171No Hit
GTTGCATCGAATTAATCCACATCATCCACCGCTTGTGCGGGTCCCCGTCA1931440.14323875109253068No Hit
CGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCGATCACGAGTATTTA1921230.14248156078444205No Hit
CCCATCTTCAGACAGGATTTCACGTGTCCCGCCCTACTTGTCGTACACCT1845010.13682896085471463No Hit
GTCCCAGTGTGGCTGATCGTCCTCTCAGACCAGCTACTGATCGTCGCCTT1822390.13515142463836155No Hit
CTCCCTCTGACACACTCTAGCCGTGCAGTCACCAATGCAATTCCCAGGTT1819850.1349630540817949No Hit
GGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCATCGAATTAATCCAC1818760.13488221789807145No Hit
CCCAGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCAC1810790.13429114965561637No Hit
CTCCCGAGTTCTCTCAAGCGCCTTAGAATACTCATCTCGCCCACCTGTGT1758290.13039766374232994No Hit
CCAGTAATTCCGATTAACGCTTGGACCCTACGTATTACCGCGGCTGCTGG1758130.130385797880499No Hit
CAGCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCC1742990.12926299070474365No Hit
GTCCCCCCATCGCATCATACGACGGTGCAGGAATATTAACCTGCTTCCCA1698960.12599765385213413No Hit
GCGGCATTGCTGGATCAGGCTTTCGCCCATTGTCCAAAATTCCCCACTGC1692220.1254978044225046No Hit
CTCATCTTCAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTT1682260.12475915452352683No Hit
CCCAACCATGACAGCGCTCTACCCCCGAAGGTGAGACACGAGGCACTACC1643200.12186240100404176No Hit
GCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTAGAATACTCATCTC1631950.12102808259405182No Hit
CCCATCGCATCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGC1615300.11979329134726671No Hit
CCGGACTACGATGCATTTTCTGGGATTAGCTCCACCTCGCGGCTTGGCAA1574430.11676230526581882No Hit
GTTCCGATGTATTACTCACCCGTTCGCCACTCGCCGGCAGGAAAGCAAGC1564260.11600808142318791No Hit
GCCATTTCTTTCCGGACAAAAGTGCTTTACAACCCGAAGGCCTTCTTCAC1524630.11306905577093002No Hit
CCACCAACTAGCTAATCAGACATCGGCCGCTCCTATAGCATGAGGCCTTG1516260.1124483228738975No Hit
CCGCAGCTTCGGTATATGGCTTAGCCCCGTTACATCTTCCGCGCAGGACG1510990.11205749104983997No Hit
CCCCAGTCAAACTGCCTACCATGCACTGTCCCCGACCCGGATTCACGGGC1508060.11184019745506038No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA1497860.11108374876333618No Hit
CCCCATCTTCAGACAGGATTTCACGTGTCCCGCCCTACTTGTCGTACACC1477190.109550827738048No Hit
CTTCGGTATATGGCTTAGCCCCGTTACATCTTCCGCGCAGGACGACTCGA1458950.10819811948931764No Hit
CATGGATAGATCACTTGGTTTCGGGTCTACACCCAGCGACTGAACGCCCT1454360.10785771757804175No Hit
CAGTAATTCCGATTAACGCTTGGACCCTACGTATTACCGCGGCTGCTGGC1453690.10780802928162456No Hit
CCCCCCATCGCATCATACGACGGTGCAGGAATATTAACCTGCTTCCCATC1422370.10548528682821257No Hit
CCCCCATCTTCAGACAGGATTTCACGTGTCCCGCCCTACTTGTCGTACAC1412150.10472735490375948No Hit
CTTCGTTTCCCACTTAGCCATATTTAGGGACCTTAGCTGGCGGTCTGGGT1398230.10369502492446525No Hit
CCCATTCGGACATCTGCGGATCAAAGCTTGTTTGCCAGCTCCCCGCAGCT1391980.10323151469669309No Hit
CTCCAATGCAGGGACCGGGACATCCAACACCCGGACAACCTTCCGCGATC1390800.10314400396568969No Hit
CTCACGGTACTGGTTCACTATCGGTCGATCACGAGTATTTAGCCTTGGAG1387540.1029022370308837No Hit
GGCATTGCTGGATCAGGCTTTCGCCCATTGTCCAAAATTCCCCACTGCTG1379080.10227482958657128No Hit
CCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGAGGCTGCCTTCGC1371060.10168005326229401No Hit
CCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGG1363090.1010889850198389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)