FastQCFastQC Report
Wed 31 May 2023
EGAF00007967471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007967471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134840606
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTGATCCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATGCAA8122820.6024016237363988No Hit
AGCAAACTCCGAATACCGAAGAGTGCAATCACGGGAGACAGACATCGGGT4859330.36037586481923706No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4772950.35396978266324314No Hit
GTGCATGTGGTGGATGCCTTGGCGATCACAGGCGATGAAGGACGTAGTAG3605420.26738384726630493No Hit
AGTGCATGTGGTGGATGCCTTGGCGATCACAGGCGATGAAGGACGTAGTA3321410.24632120089997223No Hit
TAGCAAACTCCGAATACCGAAGAGTGCAATCACGGGAGACAGACATCGGG2836070.21032759226845954No Hit
GTCTGATTAGCTAGTTGGTGGGGTAAAGGCCTACCAAGGCGACGATCAGT2496560.1851489750795098No Hit
CAGACATCGGGTGCTAACGTCCGGTGTCAAGAGGGAAACAACCCAGACCG2225210.16502521503055242No Hit
GGGCGAGCGACTTTAGGTCGCGAAGCAATTGGAAGTGGTTCCAAGAAAAG2044840.15164868066522927No Hit
CAAACTCCGAATACCGAAGAGTGCAATCACGGGAGACAGACATCGGGTGC1979080.1467718114527014No Hit
GTGAAATCCTGTCTGAAGATGGGGGGACCATCCTCCAAGGCTAAATACTC1928360.14301033325228454No Hit
GGAAAGTCCGGCCATAGTGGGTGATAGCCCCGTATGCGAAAACAGTATTG1910090.14165540015446088No Hit
CCTGGCTCAGATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAAC1861940.13808451735970395No Hit
GTGGGTGATAGCCCCGTATGCGAAAACAGTATTGTGGAACTAGGTGTACG1791430.13285538037406922No Hit
GCAAACTCCGAATACCGAAGAGTGCAATCACGGGAGACAGACATCGGGTG1660590.1231520718617951No Hit
GCAAGACCGATGTGAAATCCCCGGGCTTAACCTGGGAATTGCATTGGTGA1522550.11291479956712742No Hit
CCCGAAACCAAGTGATCTATCCATGGCCAGGTTGAAGGTGCGGTAACACG1461590.1083939062095286No Hit
ATTGAACGCTGGCGGCATGCCTTACACATGCAAGTCGAACGGCAGCGGGG1448000.10738604957026075No Hit
CGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCAGGCGGTTGTGCAAGA1439980.10679127324598348No Hit
CTAGTCGAAAGATTAGCTAATACCGCATACGACCTGAGGGTGAAAGTGGG1436750.10655173116027082No Hit
GCAAGGCCTCATGCTATAGGAGCGGCCGATGTCTGATTAGCTAGTTGGTG1436620.10654209014753316No Hit
CGGCGTACCTTTTGTATAATGGGTCAGCGACTTACATTCAGTGGCAAGCT1435280.10644271355469881No Hit
GCGACTTACATTCAGTGGCAAGCTTAACCGATTAGGGAAGGCGTAGCGAA1411210.10465764296550253No Hit
GTGGGGATGACGTCAAGTCCTCATGGCCCTTATGGGTAGGGCTTCACACG1349680.10009447747513091No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)