FastQCFastQC Report
Thu 1 Jun 2023
EGAF00007967840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007967840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221216416
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGATTTCTGTTCCTGCAGCTACTTAGATGTTTCAGTTCACTGCGTTCG9628750.4352638097165447No Hit
GTCAGCATTCGCACTTCTGATACCTCCAGCATCCTTTACAAGACACCTTC7191340.32508166120908494No Hit
CCCCATTCGGACATCTGCGGATCAAAGCTTGTTTGCCAGCTCCCCGCAGC6367500.2878403020506399No Hit
GTTCGCTTCTGTTACCTATGTATTCAGTAACAGATGACCCATACGGGCCG5359130.2422573377194575No Hit
CCTCACGGTACTGGTTCACTATCGGTCGATCACGAGTATTTAGCCTTGGA5252950.23745751309884708No Hit
CTTGGTTTCGGGTCTACACCCAGCGACTGAACGCCCTATTCGGACTCGCT5193010.23474794926611597No Hit
GCGGTTTCAGGATCTATTTCACTCCCCTCCCGGGGTTCTTTTCGCCTTTC4987430.225454787225194No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT4904840.2217213391613758No Hit
CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG4495720.20322723246723245No Hit
GCCATGGATAGATCACTTGGTTTCGGGTCTACACCCAGCGACTGAACGCC4415160.19958554974509665No Hit
GTCCCGCCCTACTTGTCGTACACCTAGTTCCACAATACTGTTTTCGCATA4301620.19445301925513522No Hit
CCCACTTTCACCCTCAGGTCGTATGCGGTATTAGCTAATCTTTCGACTAG4264140.19275875077914653No Hit
CCCCATCGCATCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAG4150250.18761039867855014No Hit
ACTGAATGTAAGTCGCTGACCCATTATACAAAAGGTACGCCGTCACCCGT3979500.1798917129188098No Hit
GCCTTTCACCCCTATCCACAGCTCATCCCCTAACTTTTCAACGTTAGTGG3863880.1746651568570752No Hit
CTCATCTTCAGGCAAGTTTCCCGCTTAGATGCTTTCAGCGGTTATCTCTT3812280.17233259940347284No Hit
GCCATATTTAGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCCTCTTGACA3804380.17197548304914226No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC3652940.16512969815043022No Hit
GTCCAATGCAAAGCTACAGTAAAGGTTCATGGGGTCTTTCCGTCTAGCCG3642070.16463832412871204No Hit
CCCATTCGGACATCTGCGGATCAAAGCTTGTTTGCCAGCTCCCCGCAGCT3623890.16381650446773355No Hit
CCCCCATCGCATCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCA3582450.1619432257685614No Hit
CTCTGACACACTCTAGCCGTGCAGTCACCAATGCAATTCCCAGGTTAAGC3555840.16074033131429089No Hit
CTCCTACCATGCACTTACGTGCATCCGCAGCTTCGGTATATGGCTTAGCC3485860.15757691327934722No Hit
GTTACATCTTCCGCGCAGGACGACTCGATCAGTGAGCTATTACGCTTTCT3482840.15744039538186894No Hit
CCCACTTCGTTTCCCACTTAGCCATATTTAGGGACCTTAGCTGGCGGTCT3461640.15648205782341215No Hit
GTCCTGCTTTCGCACCTGCTCGACTTGTCGGTCTCGCAGTTAAGCACGCT3447940.15586275477856038No Hit
CAGCTATTTCCAAGTTTGTTTAGCCTTTCACCCCTATCCACAGCTCATCC3314100.14981257087177471No Hit
GTCGATCACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATCTTCAGACA3191760.14428223988585007No Hit
CCCAGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCAC3186630.14405034027854424No Hit
GGATAGATCACTTGGTTTCGGGTCTACACCCAGCGACTGAACGCCCTATT3106260.14041724643075315No Hit
GGGCTTACGGCGAGGGGGCCTTTCACCCCCTTTATCGCTACTCATGTCAG3060760.13836043704821618No Hit
GCCGAGTTCCTTCTCCCGAGTTCTCTCAAGCGCCTTAGAATACTCATCTC3022410.1366268405686493No Hit
CCCCACTTTCACCCTCAGGTCGTATGCGGTATTAGCTAATCTTTCGACTA3016610.1363646538781281No Hit
CCCGTTACATCTTCCGCGCAGGACGACTCGATCAGTGAGCTATTACGCTT3014570.13627243649042756No Hit
CTTACGGCGAGGGGGCCTTTCACCCCCTTTATCGCTACTCATGTCAGCAT2985120.13494116096700529No Hit
GGCATGTCAAGGGTAGGTAAGGTTTTTCGCGTTGCATCGAATTAATCCAC2956390.13364243275688908No Hit
CCCATCGCATCATACGACGGTGCAGGAATATTAACCTGCTTCCCATCAGC2937090.1327699839418789No Hit
GTCCTGGTCTCGAACGACCCTTCAGGGAGATCAAGTCTCCAGGGAATCCT2872790.12986332804523876No Hit
CCCCAGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCA2828180.12784675075831622No Hit
GCCATTTCTTTCCGGACAAAAGTGCTTTACAACCCGAAGGCCTTCTTCAC2811710.12710223096643966No Hit
CTCCAATGCAGGGACCGGGACATCCAACACCCGGACAACCTTCCGCGATC2679610.12113070306681037No Hit
GGTCGATCACGAGTATTTAGCCTTGGAGGATGGTCCCCCCATCTTCAGAC2648570.11972755222650383No Hit
GCTAACAATACTGCTAAAGAGTACAAGGCTGATCCCATTTCGCTCGCCAC2628720.11883024088049596No Hit
GCCATCTCCAATGCAGGGACCGGGACATCCAACACCCGGACAACCTTCCG2623430.1185911085368999No Hit
CTCGGTTGATTTCTGTTCCTGCAGCTACTTAGATGTTTCAGTTCACTGCG2604670.11774307020686928No Hit
CTCCTGACTGTTTTAGCCTTCCCACTTCGTTTCCCACTTAGCCATATTTA2585840.11689186755471166No Hit
CCCCACTACAGGGCACGTTCCGATGTATTACTCACCCGTTCGCCACTCGC2578680.11656820260572344No Hit
CCCATCTTCAGACAGGATTTCACGTGTCCCGCCCTACTTGTCGTACACCT2544240.1150113561192493No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA2513030.11360052049663438No Hit
GGGGTCTTTCCGTCTAGCCGCGGGGAGATTGCATCATCACAAACACTTCA2482870.11223714970592417No Hit
CTCAGGTCGTATGCGGTATTAGCTAATCTTTCGACTAGTTATCCCCCACT2403290.10863976749356612No Hit
CCCACTTAGCCATATTTAGGGACCTTAGCTGGCGGTCTGGGTTGTTTCCC2366320.10696855336450256No Hit
CCGGACAACCTTCCGCGATCCGTCCCCCCATCGCATCATACGACGGTGCA2358100.1065969715375915No Hit
CCGGACAAAAGTGCTTTACAACCCGAAGGCCTTCTTCACACACGCGGCAT2357650.1065766294667752No Hit
GCACCATTGTATGACGTGTGAAGCCCTACCCATAAGGGCCATGAGGACTT2317630.10476754130217895No Hit
CCTGACTGTTTTAGCCTTCCCACTTCGTTTCCCACTTAGCCATATTTAGG2298040.1038819831526427No Hit
GGCCGACTCACCCTACGCCGATGAACGTTGCGTAGGAAACCTTGGGCTTA2278860.1030149588898502No Hit
GCCACTGAATGTAAGTCGCTGACCCATTATACAAAAGGTACGCCGTCACC2249680.10169588860891769No Hit
CCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGG2237170.10113037904022457No Hit
GTCCCCCCATCGCATCATACGACGGTGCAGGAATATTAACCTGCTTCCCA2223760.10052418532989883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)