FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007973358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007973358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49614537
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGTAAA1298156426.164839550956607No Hit
ACCAGTCC1283785225.87518250951329No Hit
CTTTCCTT1216948724.52806724771008No Hit
GGACAGGG1078402221.735609464621223No Hit
CTTTCCTA1044850.210593520201549No Hit
GGACAGGA732230.14758376158987435No Hit
ACCAGTCA681260.13731056282959972No Hit
GGCCAGGG511820.10315928172422531ABI Solid3 GAPDH Forward Primer (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)