FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007973370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007973370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47841600
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGTAAA1249770226.12308534831611No Hit
ACCAGTCC1235733025.829675428915422No Hit
CTTTCCTT1170167524.459204959700344No Hit
GGACAGGG1037762621.691636567338886No Hit
CTTTCCTA1031110.21552581853449718No Hit
GGACAGGA708900.14817648239189324No Hit
ACCAGTCA673020.14067673321962476No Hit
GGCCAGGG629570.13159467910772216ABI Solid3 GAPDH Forward Primer (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)