FastQCFastQC Report
Thu 6 Jun 2024
EGAF00007977170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007977170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125775469
Total Bases18.9 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAACTGATCTCGTATG8035890.6389075758485147TruSeq Adapter, Index 7 (97% over 36bp)
NTCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAACTGATCTCGTATG3540860.28152230543461537TruSeq Adapter, Index 2 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)