FastQCFastQC Report
Wed 31 May 2023
EGAF00007981090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007981090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39608745
Sequences flagged as poor quality0
Sequence length35-101
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC6424161.621904455695327No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA5157361.3020760945594212No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC4463541.1269077068712983No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC3774600.9529713703375353No Hit
CTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAAC3189900.8053524543632978No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG2779510.7017414967326029No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA2777660.701274428159741No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT2347760.5927377906065946No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2310740.5833913697593802No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA2299940.5806646991718621No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA2232400.563612909219921No Hit
CTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTG2162100.5458643034511697No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT2053620.5184764122165446No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC2019480.5098571035260017No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCG1567960.3958620754078424No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC1381220.34871592119366573No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1237490.3124284801247805No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT1131660.28570963306209274No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC1081310.2729977937952844No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAG1063840.26858715165047514No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGG1060760.26780954559403486No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA977500.24678893512026195No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC922120.23280717427426695No Hit
GTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCAC921830.23273395811960212No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC896590.22636162796877304No Hit
CTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCACGTG838450.2116830513059679No Hit
CACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCG826270.20860797281004487No Hit
CTCGGGATCCCACCTCGGCCGGCGAGCGCGCCGGCCTTCACCTTCATTGC826260.20860544811505644No Hit
CGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCC811390.20485122666724231No Hit
GGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGG804620.20314200816006667No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCG780830.1971357587825618No Hit
CTTGTTGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTAC773600.19531040430591778No Hit
CGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGAC770470.1945201747745353No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT768710.19407582845656937No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT767570.1937880132278869No Hit
TGACTATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCC742870.18755201660643375No Hit
CCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTC724840.18299999154227178No Hit
CTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGG711690.1796800176324698No Hit
CGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCT681590.17208068571725765No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC675780.1706138379289725No Hit
GGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACG657740.1660592881698221No Hit
GTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGG638750.16126489238676964No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT628200.1586013391739627No Hit
CTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCG624460.1576571032482852No Hit
CGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCG613610.15491780918582498No Hit
GGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTT606180.15304196080941215No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT577930.14590969746706187No Hit
ATCGGTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTT575080.14519015939535576No Hit
GGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTT559220.14118599314368582No Hit
CCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCA553060.13963078103080517No Hit
CTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGG547800.1383027914668844No Hit
CCCGCCGCAGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAG542680.13701014763280178No Hit
CTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAA534570.13496261999717485No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC528600.13345537708907465No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT528440.13341498196925958No Hit
CTCTGGCTTCGCCCTGCCCAGGCATAGTTCACCATCTTTCGGGTCCTAAC517830.13073628058652198No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC489870.12367723339883656No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT489290.12353080108950687No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA477370.12052136466328334No Hit
CCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAG476490.12029919150430039No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC468390.11825418856366188No Hit
GGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGG467570.11804716357460959No Hit
CTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCG466090.11767350871632008No Hit
GACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGC463660.11706000783412854No Hit
CACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAC452160.11415660859741958No Hit
AGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCC451350.11395210830335574No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC451070.11388141684367935No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG447990.11310381078723902No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA441300.11141478983997094No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA439280.11090480145230554No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG438650.11074574566803366No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT435380.10992017040681296No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT434330.10965507743302648No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA422840.10675420289130595No Hit
CCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGC421770.1064840605275426No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC410280.10358318598582207No Hit
CCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGC407230.10281315401434708No Hit
CGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCC405620.10240667812120782No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA403600.10189668973354243No Hit
CGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCAC403380.10184114644379669No Hit
GTCTCGTGCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGC400160.10102819465751818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)