FastQCFastQC Report
Sun 19 May 2024
EGAF00007981335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007981335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3475311
Total Bases12.4 Gbp
Sequences flagged as poor quality0
Sequence length26-266110
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG243360.7002538765595366No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT106570.30664881502691416No Hit
ATTGTACTTCGTTCAGTTACGTATTGCTGGCCTACACGACGCTCTTCCGA61740.17765316542893572Illumina Single End Sequencing Primer (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52840.15204394657053713No Hit
TTGTACTTCGTTCAGTTACGTATTGCTGGCCTACACGACGCTCTTCCGAT43400.12488090993870765Illumina Single End PCR Primer 1 (95% over 22bp)
GATGTACTTCGTTCAGTTACGTATTGCTGGCCTACACGACGCTCTTCCGA42650.12272282969783135Illumina Single End PCR Primer 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)