FastQCFastQC Report
Thu 16 May 2024
EGAF00007981339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007981339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333833
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length114-263958
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGTACTTCGTTCAGTTACGTATTGCTGGCCTACACGACGCTCTTCCGA5100.15277099627658142Illumina Single End Sequencing Primer (95% over 21bp)
AGTGTACTTCGTTCAGTTACGTATTGCTGGCCTACACGACGCTCTTCCGA3360.10064912695868893Illumina Single End Sequencing Primer (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)