FastQCFastQC Report
Sun 19 May 2024
EGAF00007981343

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007981343
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3642331
Total Bases17.6 Gbp
Sequences flagged as poor quality0
Sequence length80-349806
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG349350.9591385296943084No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT142560.3913977065785619No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90640.24885162825673995No Hit
ATTGTACTTCGTTCAGTTACGTATTGCTGGCCTACACGACGCTCTTCCGA43160.11849554584687663Illumina Single End Sequencing Primer (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41080.11278491713136451No Hit
TTGTACTTCGTTCAGTTACGTATTGCTGGCCTACACGACGCTCTTCCGAT37620.10328550590267606Illumina Single End PCR Primer 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)