FastQCFastQC Report
Wed 15 May 2024
EGAF00007981357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007981357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4112182
Total Bases18.8 Gbp
Sequences flagged as poor quality0
Sequence length72-221239
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG674341.639859325292509No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT293980.7149002646283652No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148660.36151123661355455No Hit
ATTGTACTTCGTTCAGTTACGTATTGCTGGCCTACACGACGCTCTTCCGA78110.18994781845745154Illumina Single End Sequencing Primer (95% over 21bp)
TTGTACTTCGTTCAGTTACGTATTGCTGGCCTACACGACGCTCTTCCGAT67330.16373302543515827Illumina Single End PCR Primer 1 (95% over 22bp)
GATGTACTTCGTTCAGTTACGTATTGCTGGCCTACACGACGCTCTTCCGA57160.1390016297916775Illumina Single End PCR Primer 1 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52320.1272317227204438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)