FastQCFastQC Report
Wed 31 May 2023
EGAF00007984464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14216704
Sequences flagged as poor quality0
Sequence length0-50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA518959936.5035313389095No Hit
AAACCGTTACCATTACTGAGTTA10315257.255725377696547No Hit
AAACCGTTACCATTACTGAGCA5078523.5722203965138477No Hit
AAACCGTTACCATTACTGAGGA5020593.5314725551013795No Hit
AAACCGTTACCATTACTGAGA3484502.4509900466380956No Hit
TGAGGTAGTAGATTGTATAGTA3066372.156878275020708No Hit
TGAGGTAGTAGATTGTATAGTTA2845532.0015398787229444No Hit
TGAGGTAGTAGTTTGTACAGTTA2079901.46299733046422No Hit
TGAGGTAGTAGTTTGTGCTGTTA1654471.1637507540425684No Hit
AAACCGTTACCATTACTGAGTTTA1401410.9857488768142039No Hit
TGAGGTAGTAGTTTGTACAGTA1288430.9062789799942378No Hit
TATTGCACTTGTCCCGGCCTGTA1279410.8999343307703389No Hit
TCCTGTACTGAGCTGCCCCGAGA1139550.8015570979039868No Hit
AAACCGTTACCATTACTGAGTCA1001100.7041716561025678No Hit
AAACCGTTACCATTACTGAGAA998810.7025608748694494No Hit
TAGCAGCACGTAAATATTGGCGA967480.6805234180862174No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA876220.6163313240537329No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA705870.4965074886555984No Hit
TGAGGTAGTAGGTTGTATAGTA549320.38639054453127814No Hit
TATTGCACTTGTCCCGGCCTGTAA500310.35191701255086977No Hit
CATTGCACTTGTCTCGGTCTGAA446540.3140953064789138No Hit
TGAGGTAGTAGGTTGTATAGTTA435100.30604843429250544No Hit
AAACCGTTACCATTACGGA429470.30208830401195663No Hit
AAACCGTTACCATTACTGAGTGA408620.28742245741347644No Hit
TGAGGTAGTAGATTGTATAGTCA390040.2743533240897468No Hit
AAACCGTTACCATTACTGAGGTA389040.2736499261713545No Hit
TGAGGTAGTAGTTTGTGCTGTA385590.2712232033529009No Hit
TCCTGTACTGAGCTGCCCCGAA374940.2637320155220225No Hit
TCCTGTACTGAGCTGCCCCGAGAA370370.2605174870349696No Hit
TGAGGTAGTAGATTGTATAGCA365320.25696532754708823No Hit
AAACCGTTACCATTACTGGGTA338270.23793841385457556No Hit
TTCAAGTAATCCAGGATAGGCTA328920.23136164331760722No Hit
TGAGGTAGTAGTTTGTACAGTCA301060.21176497731119673No Hit
TCCTGTACTGAGCTGCCCCGAGTA290070.20403463418806497No Hit
AAACCGTTACCATTACTGAGTAA288150.20268411018475166No Hit
CAACGGAATCCCAAAAGCAGCTA286770.20171342105737025No Hit
AAACCGTTACCATTACTGAAA285820.20104519303489754No Hit
TGAGGTAGTAGATTGTATAGGA282270.19854813042460476No Hit
TGAGGTAGTAGGTTGTGTGGTA261260.18376974015918177No Hit
AAACCGTTACTATTACTGAGTA260890.1835094829293766No Hit
TATTGCACTTGTCCCGGCCTGTTA252440.17756577051896136No Hit
AAACCGTTACCATTACTGCGA245130.17242393173551337No Hit
TGAGGTAGTAGTTTGTGCTGTCA239190.1682457481002629No Hit
TAGCTTATCAGACTGATGTTGAA238990.16810506851658444No Hit
AAACCGTTACCATTACTGA235320.16552359815608456No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA229510.16143685625022508No Hit
TAGCAGCACGTAAATATTGGCAA223110.15693510957251414No Hit
AAACCGTTACCATTACTGAGTTTAGTA223040.15688587171822668No Hit
TACAGTACTGTGATAACTGAAGA215700.15172293099722692No Hit
TAGCAGCACGTAAATATTGGCA215210.15137826601721469No Hit
AAACCGTTACCATTACTGACTA212200.14926103828285375No Hit
TCCTGTACTGAGCTGCCCCGA211110.1484943345518061No Hit
TGAGGTAGTAGATTGTATAGA209930.14766432500810314No Hit
AAACCGTTACCATTACTGAGCTA206490.1452446361688335No Hit
TAAAGTGCTTATAGTGCAGGTAGA202990.14278274345446032No Hit
AAACCGTTACCATTACTGAGGGA202860.14269130172506933No Hit
TATTGCACTTGTCCCGGCCTGCA200480.14101721467929557No Hit
AGACCGTTACCATTACTGAGTA189540.1333220414520834No Hit
AGCAGCATTGTACAGGGCTAGGA186490.1311766778009868No Hit
AAACCGTTACCATTACCGAGTA176420.12409346076277594No Hit
TATTGCACTTGTCCCGGCCTGGA170800.12014036446141103No Hit
TGAGGTAGTAGATTGTATA167600.11788949112255555No Hit
GAACCGTTACCATTACTGAGTA166730.11727753493355422No Hit
TCGTACCGTGAGTAATAATGCGA166160.11687659812007059No Hit
AAACCGTTACCATTACTGCGGA165780.1166093069110815No Hit
TGAGGTAGTAGTTTGTGCTGA162070.11399970063384593No Hit
AAACCGTTACCATTA162000.11395046277955848No Hit
TGAGGTAGTAGGTTGTGTGGTTA162000.11395046277955848No Hit
TCAGTGCACTACAGAACTTTGTA161030.1132681667987179No Hit
TAGCAGCACGTAAATATTGGA160680.11302197752728058No Hit
TGAGGTAGTAGTTTGTACAGCA155890.10965270149818131No Hit
TCCTGTACTGAGCTGCCCCGCGA149020.10482035779882594No Hit
TGGAGAGAAAGGCAGTTCCTGAA147810.10396924631757122No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGA146100.10276643587712032No Hit
AGCAGCATTGTACAGGGCTATGAA145520.10235846508445277No Hit
CATTGCACTTGTCTCGGTCGGA145110.1020700719379119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)