FastQCFastQC Report
Sun 21 May 2023
EGAF00007984465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9743688
Sequences flagged as poor quality0
Sequence length0-50
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAGCTGGGTTGAGAGGGCGAA8768388.999036093930759No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA6063326.22281830042177No Hit
AAAAGCTGGGTTGAGAGGGCGA3208753.293157580579345No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCAA3201903.286127388315389No Hit
AAAAGCTGGGTTGAGAGGGCGAAA2999353.0782492214446933No Hit
A2268042.3277017901230006No Hit
TGAGGTAGTAGGTTGTGTGGTA1872341.9215927275175477No Hit
AAAAGCTGGGTTGAGAGGGCAA1746441.792380872622358No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGA1741791.787608552326388No Hit
TGAGGTAGTAGGTTGTGTGGTTA1723931.7692787371681031No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCA1151131.1814109811397902No Hit
TCCTGTACTGAGCTGCCCCGAGA1046721.0742544301500623No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCTA906920.9307769296389622No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCAA871860.8947946609127878No Hit
ACTGGACTTGGAGTCAGAAGGA781540.8020987535725693No Hit
CGGGGCTGGGCGA769130.7893623030622491No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCGCGTATG706230.724807690886654TruSeq Adapter, Index 16 (97% over 42bp)
AGGGCTGGGTCGGTCGGGCTGGGGTA633600.6502671267799215No Hit
TGGAGAGAAAGGCAGTTCCTGAA631240.6478450459415367No Hit
AAAAGCTGGGTTGAGAGGGCGTA601540.6173637743737279No Hit
ACTGGACTTGGAGTCAGAAGGAA589980.6054996834874024No Hit
AGCGGGGCTGGGCGA576220.5913777206330908No Hit
TGAGGGGCAGAGAGCGAGACTA523510.5372811608910302No Hit
AAACCGTTACCATTACTGAGTA495150.5081751386128127No Hit
TCCTGTACTGAGCTGCCCCGAA487820.5006523197376599No Hit
TCGGGCTGGGGCGA484460.49720393345928154No Hit
TGAGGTAGTAGGTTGTGTGGCA482860.49556184475529186No Hit
TGAGGTAGTAGGTTGTGTGGTCA456120.46811843728986396No Hit
GGGATTGTGGGTA425380.43656980806446183No Hit
CGGGGCTGGGCAA422060.43316247400368324No Hit
TCCTGTACTGAGCTGCCCCGAGAA417690.42867751923091135No Hit
AAGCGGGGCTGGA415950.42689174776532257No Hit
AAAAGCTGGGTTGAGAGGGCGAAAA410410.4212060156277582No Hit
AAAAGCTGGGTTGAGAGGGCTA407690.4184144648309757No Hit
AAAAGCTGGGTTGAGAGGGCGGA406660.41735737022778235No Hit
AAGCGGGGCTA362940.37248729639126377No Hit
TCCTGTACTGAGCTGCCCCGA346940.35606640935136674No Hit
AAGCGGGGCTGA325520.33408294682670464No Hit
CGGGGCTGGGCA314120.322383064810778No Hit
AGCGGGGCTGGGCAA303980.31197632764924327No Hit
AAGCGGGGCTGGGCGCGA292520.30021486730691704No Hit
AGGGCTGGGTCGGTCGGGCTGGA282630.2900647065053807No Hit
TCGGGCTGGGGCAA279990.28735526014379775No Hit
ACTGGACTTGGAGTCAGAAGAA269840.27693825992786303No Hit
AAGCTGCCAGTTGAAGAACTGTA268000.27504985791827485No Hit
AGCGGGGCTA262970.26988754155510725No Hit
TCCTGTACTGAGCTGCCCCGAAA253890.2605686881599657No Hit
AGGGCTGGGTCGGTCGGGCTGGGA249190.255745052591996No Hit
GCGGGGCTGGGCGA235050.24123309367048698No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCA232520.2386365409073033No Hit
AGCGGGGCTGGA230360.23641972115691717No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCA228050.23404895559053204No Hit
AGCGGGGCTGA226750.2327147585185404No Hit
AGCGGGGCTGGGA225400.2313292461745491No Hit
AGGGCTGGGTCGGTCGGGCTGA220260.2260540362129822No Hit
CGGGGCTGGGTA203820.209181574779488No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCCA202110.20742659247709902No Hit
AAGCGGGGCTGGGCGA194020.1991237814675511No Hit
GGCTGGGCGA191780.19682485728196553No Hit
AAGGGCTGA191760.19680433117316565No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGTA189660.19464908974917916No Hit
TGAGGGGCAGAGAGCGAGACTTA186080.1909749162740022No Hit
ACTGGACTTGGAGTCAGAAGA183010.18782415857322196No Hit
TCCTGTACTGAGCTGCCCCAA182570.1873725841796248No Hit
AAAAGCTGGGTTGAGAGGGCA181490.18626417430443173No Hit
TGGAGAGAAAGGCAGTTCCTGA177610.18228210919725674No Hit
TGGGTTTACGTTGGGAGAACTA176510.1811531732132638No Hit
AAGCGGGGCTGGGA175350.17996265890287128No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCTA174370.17895687957167758No Hit
AAAAGCTGGGTTGAGAGGGCGAGA172450.17698637312688995No Hit
AAAAGCTGGGTTGAGAGGGCGATA156300.16041154027099389No Hit
AAGCGGGGCTGGGCGCAA152450.15646026432701868No Hit
AAAGCTGGGTTGAGAGGGCGAA152280.15628579240221976No Hit
AAACCGTTACCATTACTGAGTTA150090.15403818348863385No Hit
CTGGGTCGGTCGGGCTGGGGCGA149850.15379187018303542No Hit
TGAGGTAGTAGGTTGTGTGGTAA149760.153699502693436No Hit
ACTGGACTTGGAGTCAGAAA146130.14997401394625937No Hit
AGGGCTGGGTCGGTCGGGCTGGGGAA144950.14876297352706694No Hit
TGAGGTAGTAGGTTGTGTGGAA144210.14800350750147173No Hit
AAGCGGGGCTGAA143550.14732614591107596No Hit
CGGGGCTGGGA141770.14549932222788742No Hit
TGAGGTAGTAGGTTGTGTGGA139320.1429848738999032No Hit
TGAGGGGCAGAGAGCGAGACCA138310.1419483054055097No Hit
AAGCGGGGCTAA135310.138869389085529No Hit
TCAGAAGGCTGCGTGTTCGAATCACGTCGGGGTA131510.13496942841355347No Hit
AAAAGCTGGGTTGAGAGGGCGAAGA130970.13441522347595697No Hit
AAGGGCTGGGTCGGTCGGGCTGGGGCGA130510.13394312297355992No Hit
GCGGGGCTGGGCAA124710.12799055142159724No Hit
TCGGGCTGGGGCA124590.12786739476879802No Hit
GAAGCGGGGCTGGA120240.12340296610482601No Hit
CGGGCTGGGGCGA118650.12177114045523625No Hit
TGAGGTAGTAGTTTGTGCTGTTA118360.12147351187763811No Hit
ACCGGGCGGAAACACCAA117660.12075509806964262No Hit
GGGATTGTGGGCA116010.11906169409365325No Hit
AAAAGCTGGGTTGAGAGGGCGCA115150.11817907141525878No Hit
ACCACAGGGTAGAACCACGGAA114650.11766591869526201No Hit
AGGTTGGTGGTA107090.10990704956891068No Hit
CGGGGCTGGGCTA105880.10866521998651846No Hit
GGCTGGGCAA105680.10845995889851973No Hit
AAGCGGGGCTGGGCAA102210.10489867902174208No Hit
CGGGCTGGGGCA101920.10460105044414393No Hit
AAAAGCTGGGTTGAGAGGGCCA101540.10421105437694639No Hit
AAAGCTGGGTTGAGAGGGCGA100720.10336948391615167No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)