FastQCFastQC Report
Mon 22 May 2023
EGAF00007984466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15592893
Sequences flagged as poor quality0
Sequence length0-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA504808032.37423613437224No Hit
AAACCGTTACCATTACTGAGTTA5818853.7317321423292005No Hit
TGAGGTAGTAGATTGTATAGTA5558883.5650087511021846No Hit
AAACCGTTACCATTACTGAGCA5166633.313451839886287No Hit
TGAGGTAGTAGATTGTATAGTTA4293122.753254319131158No Hit
AAACCGTTACCATTACTGAGA3744772.401587697677397No Hit
TGAGGTAGTAGTTTGTACAGTTA3607712.3136886785537487No Hit
TGAGGTAGTAGTTTGTGCTGTTA3067671.9673514081062444No Hit
TGAGGTAGTAGTTTGTACAGTA2736901.7552227158872957No Hit
TCCTGTACTGAGCTGCCCCGAGA1894361.2148868077270845No Hit
TATTGCACTTGTCCCGGCCTGTA1878661.2048181181003421No Hit
AAACCGTTACCATTACTGAGGA1382130.8863845855929364No Hit
TGAGGTAGTAGGTTGTATAGTA1380090.8850762972592706No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA1050300.67357609649473No Hit
CATTGCACTTGTCTCGGTCTGAA1038790.6661945284944878No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA969940.6220397972332652No Hit
TGAGGTAGTAGGTTGTATAGTTA955070.6125034013893381No Hit
TGAGGTAGTAGTTTGTGCTGTA825380.5293308945299631No Hit
AAACCGTTACCATTACTGAGAA770600.49419950486417114No Hit
AAACCGTTACCATTACTGA696690.4467997054812087No Hit
TGAGGTAGTAGATTGTATAGA676440.4338130198161432No Hit
TGAGGTAGTAGATTGTATAGCA649340.41643330714832716No Hit
TCCTGTACTGAGCTGCCCCGAA648710.4160292769276362No Hit
TGAGGTAGTAGTTTGTGCTGA641240.41123863288230095No Hit
AAACCGTTACCATTACTGAGTCA632740.40578743149202656No Hit
AGCAGCATTGTACAGGGCTATGA626420.40173430292890483No Hit
TATTGCACTTGTCCCGGCCTGTAA604590.38773433512305894No Hit
TCCTGTACTGAGCTGCCCCGAGAA590440.3786596881027786No Hit
TGAGGTAGTAGATTGTATAGTCA589280.3779157594424588No Hit
TGAGGTAGTAGGTTGTGTGGTA573520.36780859074707944No Hit
TGAGGTAGTAGTTTGTACAGTCA538900.3456061681433971No Hit
AAACCGTTACCATTACTGAGTTTA532460.34147608144300096No Hit
TGAGGTAGTAGATTGTATA521830.33465887311610487No Hit
AGCAGCATTGTACAGGGCTATGAA518160.33230523675112755No Hit
TAGCAGCACGTAAATATTGGCGA510070.3271169756632076No Hit
TGGAGAGAAAGGCAGTTCCTGAA494860.3173625317636695No Hit
TACAGTACTGTGATAACTGAAGA449210.2880863737088428No Hit
TGAGGTAGTAGTTTGTGCTGTCA447840.28720776830829275No Hit
CAACGGAATCCCAAAAGCAGCTA432520.2773827794495864No Hit
CATTGCACTTGTCTCGGTCTGA419720.26917391147364383No Hit
TAGCTTATCAGACTGATGTTGAA332840.2134562200869332No Hit
TGAGGTAGTAGGTTGTGTGGTTA330220.21177596742310745No Hit
TTCAAGTAATCCAGGATAGGCTA329930.21158998525802747No Hit
TGAGGTAGTAGTTTGTACAGCA329770.2114873744083282No Hit
AAACCGTTACCATTACTGAAA317850.20384286610573163No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA297090.19052910835724968No Hit
TATTGCACTTGTCCCGGCCTGCA286870.18397484033270797No Hit
TCCTGTACTGAGCTGCCCCGAGTA284100.18219838999728916No Hit
ACCACAGGGTAGAACCACGGAA283250.18165326985826172No Hit
AATTGCACGGTATCCATCTGTAA280580.17994095130390492No Hit
TATTGCACTTGTCCCGGCCTGTTA272870.17499639098402073No Hit
TAAAGTGCTTATAGTGCAGGTAGA263600.16905137487956853No Hit
TGAGGTAGTAGTTTGTGCTA253540.16259971770472614No Hit
TCCTGTACTGAGCTGCCCCGA253040.16227905879941587No Hit
AAACCGTTACTATTACTGAGTA252680.16204818438759247No Hit
AAACCGTTACCATTACTGGGTA241030.15457683189386345No Hit
TGAGGTAGTAGGTTGTATAGA238420.1529029924081439No Hit
TCAGTGCACTACAGAACTTTGTA236100.1514151350875043No Hit
GTACAGTACTGTGATAACTGAA225540.14464281900735163No Hit
TAGCAGCACGTAAATATTGGCA225220.14443759730795305No Hit
TGAGGTAGTAGTTTGTACAGA215230.13803083237985408No Hit
CAAAGTGCTGTTCGTGCAGGTAGA213030.13661993319648894No Hit
TGAGGTAGTAGTTTGTACA210670.1351064231634245No Hit
TGAGGGGCAGAGAGCGAGACTA210410.1349396805326632No Hit
AGACCGTTACCATTACTGAGTA210390.13492685417645076No Hit
TCCTGTACTGAGCTGCCCCGAAA208780.13389433250135174No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA205470.13177157054819783No Hit
TACAGTACTGTGATAACTGAA204600.13121362405295797No Hit
TACAGTATAGATGATGTACTA199030.1276414838478017No Hit
TACCACAGGGTAGAACCACGGAA199020.12763507066969548No Hit
AATTGCACGGTATCCATCTGTA186830.11981740655823137No Hit
TCGTACCGTGAGTAATAATGCGA186490.11959935850262039No Hit
TGAGGTAGTAGTTTGTACAA185480.11895162751389367No Hit
TATTGCACTTGTCCCGGCCTGA178920.11474458267622308No Hit
GAACCGTTACCATTACTGAGTA178890.11472534314190445No Hit
AGAGGTAGTAGGTTGCATAGTA174180.11170473625388182No Hit
TAGCAGCACGTAAATATTGGA173960.11156364633554532No Hit
TACAGTACTGTGATAACTGAAA173250.11110831069000474No Hit
AAACCGTTACCATTACTGAA173190.1110698316213675No Hit
CATTGCACTTGTCTCGGTCTGTA170990.10965893243800237No Hit
TAGCTTATCAGACTGATGTTGA167800.10761312862212291No Hit
CCGCACTGTGGGTACTTGCTA166580.10683072089316588No Hit
TGAGGTAGTAGGTTGTATAGCA162020.10390631167673632No Hit
AAACCGTTACCATTACCGAGTA161450.10354076052468263No Hit
TGAGGTAGTAGATTGTATAGGTA157810.10120636369402394No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)