FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007984467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10809229
Sequences flagged as poor quality0
Sequence length0-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA357195833.04544662713687No Hit
AAACCGTTACCATTACTGAGTTA5741985.312108754472682No Hit
AAACCGTTACCATTACTGAGCA4538604.198819360751817No Hit
TGAGGTAGTAGATTGTATAGTA2479152.2935493364050297No Hit
TGAGGTAGTAGTTTGTGCTGTTA2423812.2423523453892966No Hit
AAACCGTTACCATTACTGAGA2311772.138700179263479No Hit
TGAGGTAGTAGTTTGTACAGTTA2190822.0268050570489344No Hit
TGAGGTAGTAGATTGTATAGTTA2149031.9881436502085392No Hit
TGAGGTAGTAGTTTGTACAGTA1516581.4030417895670448No Hit
TCCTGTACTGAGCTGCCCCGAGA1394081.2897127075390853No Hit
TATTGCACTTGTCCCGGCCTGTA1260711.166327404109951No Hit
AAACCGTTACCATTACTGAGGA1009110.9335633466549743No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA912210.8439177299324493No Hit
AAACCGTTACCATTACTGAGTCA744790.6890315673763596No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA725450.6711394494463944No Hit
AAACCGTTACCATTACTGAGTTTA690940.639213028052232No Hit
TGAGGTAGTAGTTTGTGCTGTA604740.5594663597190882No Hit
TGAGGTAGTAGGTTGTATAGTA593180.5487717949171028No Hit
AAACCGTTACCATTACTGAGAA571940.5291219197964998No Hit
TCCTGTACTGAGCTGCCCCGAA563720.5215173071085828No Hit
AAACCGTTACCATTACTGA551510.5102214043203266No Hit
CATTGCACTTGTCTCGGTCTGAA524630.48535376574962No Hit
TCCTGTACTGAGCTGCCCCGAGAA441570.40851202245784596No Hit
TGAGGTAGTAGGTTGTATAGTTA440810.4078089195815909No Hit
TGAGGTAGTAGTTTGTGCTGTCA433530.40107393413535786No Hit
TGAGGTAGTAGTTTGTACAGTCA400690.37069248879822975No Hit
TATTGCACTTGTCCCGGCCTGTAA385490.3566304312731278No Hit
TGAGGTAGTAGGTTGTGTGGTA383010.3543360955716638No Hit
TGAGGTAGTAGATTGTATAGTCA371380.34357677129423386No Hit
TGAGGTAGTAGATTGTATAGCA370770.34301243872250275No Hit
TGAGGTAGTAGTTTGTGCTGA351950.32560139118155423No Hit
AGCAGCATTGTACAGGGCTATGA314200.29067753120967277No Hit
TAGCAGCACGTAAATATTGGCGA294970.2728871781696918No Hit
TCCTGTACTGAGCTGCCCCGAGTA269740.24954601294874965No Hit
TACAGTACTGTGATAACTGAAGA249720.23102480297160882No Hit
TACAGTATAGATGATGTACTA241320.223253665918263No Hit
TCCTGTACTGAGCTGCCCCGA240770.22274484146834156No Hit
TATTGCACTTGTCCCGGCCTGCA237890.22008045162148013No Hit
TGAGGTAGTAGATTGTATA235160.21755483207914275No Hit
TGAGGTAGTAGTTTGTACAGCA231170.2138635419788035No Hit
CATTGCACTTGTCTCGGTCTGA226590.20962642201400303No Hit
TAGCTTATCAGACTGATGTTGAA220220.2037333097485491No Hit
AAACCGTTACCATTACTGGGTA215350.19922790052833553No Hit
AAACCGTTACCATTACTGAAA213550.19756265687404717No Hit
TGGAGAGAAAGGCAGTTCCTGAA212280.19638773496241038No Hit
TGAGGTAGTAGGTTGTGTGGTTA211380.19555511313526616No Hit
TCAGTGCACTACAGAACTTTGTA208740.1931127557756432No Hit
TGAGGTAGTAGATTGTATAGA207630.19208585552216537No Hit
AATTGCACGGTATCCATCTGTAA207140.19163253919405354No Hit
AGCAGCATTGTACAGGGCTATGAA200630.18560990797771051No Hit
TTCAAGTAATCCAGGATAGGCTA200300.18530461330775766No Hit
CAACGGAATCCCAAAAGCAGCTA196080.18140054207381487No Hit
TATTGCACTTGTCCCGGCCTGTTA184530.1707152286254644No Hit
AATTGCACGGTATCCATCTGTA179440.16600628962528224No Hit
TCCTGTACTGAGCTGCCCCGAAA174250.16120483708875075No Hit
AAACCGTTACTATTACTGAGTA168960.15631087101586988No Hit
ACCACAGGGTAGAACCACGGAA167330.15480290037337538No Hit
AGACCGTTACCATTACTGAGTA160490.1484749744870795No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA156800.14506122499578833No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCA155330.1437012760114528No Hit
AAACCGTTACCATTACTGAGGTA152530.14111089699367088No Hit
TGAGGTAGTAGTTTGTGCTA146470.13550457669089996No Hit
AAACCGTTACCATTACTGAGTAA135990.12580915808148757No Hit
GAACCGTTACCATTACTGAGTA132350.12244166535837107No Hit
TACAGTACTGTGATAACTGAA128010.11842657788080908No Hit
TGAGGTAGTAGTTTGTACA127040.11752919657822034No Hit
GTACAGTACTGTGATAACTGAA127000.11749219116368059No Hit
TAAAGTGCTTATAGTGCAGGTAGA124860.11551240148580441No Hit
TGAGGTAGTAGTTTGTGCTGGTA123510.11426346874508812No Hit
TACCACAGGGTAGAACCACGGAA123410.11417095520873877No Hit
TAGCTTATCAGACTGATGTTGA121020.11195988168998917No Hit
AAACCGTTACCATTACTGAA119940.11096073549741614No Hit
AAACCGTTACCATTACCGAGTA119010.11010035960936715No Hit
TGAGGTAGTAGTTTGTGCTGTAA117330.10854613219869799No Hit
CAAAGTGCTGTTCGTGCAGGTAGA114820.10622404243632919No Hit
TAGCAGCACGTAAATATTGGCA114690.10610377483907502No Hit
AAACCGTTACCATTACTAA114150.10560420174278849No Hit
CATTGCACTTGTCTCGGTCTGTA111900.10352264717492803No Hit
AAACCGTTACCATTA111320.10298606866410176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)