FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007984468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14128485
Sequences flagged as poor quality0
Sequence length0-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA427968730.291195411256055No Hit
AAACCGTTACCATTACTGAGTTA5394223.8179748217873324No Hit
AAACCGTTACCATTACTGAGCA5111123.6175994807652767No Hit
TGAGGTAGTAGTTTGTGCTGTTA3075412.1767443572329235No Hit
TGAGGTAGTAGATTGTATAGTA2977472.107423407392937No Hit
TGAGGTAGTAGTTTGTACAGTTA2725471.9290603344944628No Hit
TGAGGTAGTAGATTGTATAGTTA2621001.855117516138496No Hit
AAACCGTTACCATTACTGAGA2442041.7284514227817065No Hit
AAACCGTTACCATTACTGAGGA2225421.575129959086201No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA2193561.5525797705840365No Hit
TCCTGTACTGAGCTGCCCCGAGA1847741.3078118425294714No Hit
TATTGCACTTGTCCCGGCCTGTA1775541.256709406564115No Hit
TGAGGTAGTAGTTTGTACAGTA1559261.1036285914590276No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA1462071.0348384840979057No Hit
TGAGGTAGTAGGTTGTATAGTA862780.6106670318862921No Hit
AAACCGTTACCATTACTGAGAA809380.5728710473911393No Hit
TGAGGTAGTAGTTTGTGCTGTA710160.5026441263872241No Hit
CATTGCACTTGTCTCGGTCTGAA694580.49161675862627874No Hit
AAACCGTTACCATTACTGAGTCA669780.4740635673251591No Hit
TGAGGTAGTAGGTTGTATAGTTA640320.45321207475536124No Hit
TCCTGTACTGAGCTGCCCCGAA596610.4222745750871378No Hit
AAACCGTTACCATTACTGAGTTTA559050.3956899837456033No Hit
AAACCGTTACCATTACTGA533560.3776484173639283No Hit
TGAGGTAGTAGTTTGTGCTGTCA527800.37357154712624885No Hit
TCCTGTACTGAGCTGCCCCGAGAA525020.37160389100459107No Hit
TATTGCACTTGTCCCGGCCTGTAA504940.3573914683704587No Hit
TGAGGTAGTAGTTTGTACAGTCA472730.33459355337815766No Hit
TGAGGTAGTAGGTTGTGTGGTA471800.33393530870436566No Hit
TAGCAGCACGTAAATATTGGCGA422260.29887139350043546No Hit
TGAGGTAGTAGATTGTATAGTCA421020.2979937339353795No Hit
TGAGGTAGTAGATTGTATAGCA401430.28412812838743856No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA360860.2554130892307278No Hit
TGAGGTAGTAGTTTGTGCTGA356130.25206524266402236No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCA342110.2421420272591152No Hit
TCAGTGCACTACAGAACTTTGTA331080.23433510386994785No Hit
AGCAGCATTGTACAGGGCTATGA329410.23315309461700956No Hit
TCCTGTACTGAGCTGCCCCGAGTA319710.22628753189036194No Hit
AATTGCACGGTATCCATCTGTAA314760.22278397152985618No Hit
TGAGGTAGTAGGTTGTGTGGTTA310790.2199740453417334No Hit
TCCTGTACTGAGCTGCCCCGA308790.2185584653980947No Hit
TATTGCACTTGTCCCGGCCTGCA308140.2180984019164121No Hit
CAACGGAATCCCAAAAGCAGCTA302460.21407815487647827No Hit
AAACCGTTACCATTACTGGGTA301660.2135119228990228No Hit
AGCAGCATTGTACAGGGCTATGAA271680.1922923795438789No Hit
TACAGTACTGTGATAACTGAAGA269790.19095465649714036No Hit
CATTGCACTTGTCTCGGTCTGA269260.1905795278120761No Hit
TATTGCACTTGTCCCGGCCTGTTA268970.19037426872024849No Hit
TGAGGTAGTAGATTGTATA267970.18966647874842915No Hit
TTCAAGTAATCCAGGATAGGCTA263860.18675746196425164No Hit
TACAGTATAGATGATGTACTA253310.1792902777615576No Hit
TAGCTTATCAGACTGATGTTGAA250970.1776340492275003No Hit
AATTGCACGGTATCCATCTGTA234110.16570071030262623No Hit
AAACCGTTACCATTACTGAAA227940.16133364617650087No Hit
AAACCGTTACTATTACTGAGTA224960.15922443206047923No Hit
TGAGGTAGTAGATTGTATAGA223070.15788670901374066No Hit
TGGAGAGAAAGGCAGTTCCTGAA218470.1546308751433717No Hit
TGAGGTAGTAGTTTGTACAGCA217750.15412126636366177No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA217270.15378152717718852No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAA217260.1537744492774703No Hit
TCCTGTACTGAGCTGCCCCGAAA212800.15061770600315602No Hit
AGACCGTTACCATTACTGAGTA204930.14504739892493781No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTA190110.13455795154257516No Hit
ACCACAGGGTAGAACCACGGAA185250.13111809227953317No Hit
TACCACAGGGTAGAACCACGGAA182060.12886024226942946No Hit
GAACCGTTACCATTACTGAGTA173410.12273785901319215No Hit
TGAGGTAGTAGTTTGTGCTA170540.12070650179407062No Hit
TGAGGGGCAGAGAGCGAGACTA168460.1192342986526864No Hit
TAAAGTGCTTATAGTGCAGGTAGA167550.11859020977833079No Hit
TAGCAGCACGTAAATATTGGCA162840.11525651901106171No Hit
TGAGGTAGTAGTTTGTGCTGTAA160920.11389756226516856No Hit
TCGGGCTGGGGCGA159750.11306944799813992No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAATA159030.11255983921843No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACGA158690.11231919062801143No Hit
TTTGGCAATGGTAGAACTCACACTA156400.11069835159254514No Hit
AAACCGTTACCATTACTGAGTGA154850.10960127713622515No Hit
TCGTACCGTGAGTAATAATGCGA154150.1091058241559516No Hit
AAACCGTTACCATTA153650.10875192917004194No Hit
TGAGGTAGTAGTTTGTGCTGTGA152660.1080512170979408No Hit
CATTGCACTTGTCTCGGTCTGTA151830.10746375142133074No Hit
TGAGGTAGTAGTTTGTGCTGGTA147880.10466798103264434No Hit
TGAGGTAGTAGATTGTATAGGA146330.10357090657632435No Hit
CAAAGTGCTGTTCGTGCAGGTAGA144930.10258000061577728No Hit
CCGCACTGTGGGTACTTGCTA144660.10238889732338606No Hit
AAACCGTTACCATTACGGA141350.10004611251666404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)