FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007984469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14505023
Sequences flagged as poor quality0
Sequence length0-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA600559841.40357447209839No Hit
AAACCGTTACCATTACTGAGA5082503.503958594205607No Hit
AAACCGTTACCATTACTGAGTTA4634813.1953137888854086No Hit
AAACCGTTACCATTACTGAGCA4312432.9730597462685857No Hit
TGAGGTAGTAGATTGTATAGTA3669452.5297788221363042No Hit
TGAGGTAGTAGATTGTATAGTTA2798081.929042098037349No Hit
TGAGGTAGTAGTTTGTACAGTTA2731261.8829752975917375No Hit
TGAGGTAGTAGTTTGTGCTGTTA2638171.8187975296557615No Hit
TCCTGTACTGAGCTGCCCCGAGA2106021.451924619492158No Hit
TGAGGTAGTAGTTTGTACAGTA1961921.3525797235895456No Hit
TATTGCACTTGTCCCGGCCTGTA1515851.045051772754859No Hit
AAACCGTTACCATTACTGAGGA1224930.8444867684801327No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA1194990.8238456429886392No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA1173450.8089956148294284No Hit
AAACCGTTACCATTACTGA917340.6324291936662216No Hit
CATTGCACTTGTCTCGGTCTGAA803520.5539598248137904No Hit
TGAGGTAGTAGGTTGTATAGTA756410.5214814206085713No Hit
TCCTGTACTGAGCTGCCCCGAA580740.40037165056546276No Hit
TATTGCACTTGTCCCGGCCTGTAA566360.3904578434656739No Hit
AGCAGCATTGTACAGGGCTATGA519320.3580276984048905No Hit
TGAGGTAGTAGTTTGTGCTGTA519140.3579036034620559No Hit
TCCTGTACTGAGCTGCCCCGAGAA507070.3495823481286448No Hit
TGAGGTAGTAGGTTGTATAGTTA501900.3460180656038946No Hit
AAACCGTTACCATTACTGAGTTTA462890.31912393382623383No Hit
TGAGGTAGTAGTTTGTGCTGA460210.317276298010696No Hit
TGAGGTAGTAGGTTGTGTGGTA435470.3002201375344251No Hit
AAACCGTTACCATTACTGAGAA412610.28446007979442706No Hit
TAGCAGCACGTAAATATTGGCGA379390.26155766867794694No Hit
TGAGGTAGTAGATTGTATAGA366120.25240911372563835No Hit
CATTGCACTTGTCTCGGTCTGA345310.23806235950125693No Hit
CAACGGAATCCCAAAAGCAGCTA342940.2364284427539343No Hit
AATTGCACGGTATCCATCTGTAA340550.23478073767963004No Hit
TGAGGTAGTAGATTGTATAGCA309370.2132847359152757No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA309190.21316064097244106No Hit
TGGAGAGAAAGGCAGTTCCTGAA302310.20841745649076182No Hit
TACAGTACTGTGATAACTGAAGA296320.20428785255976498No Hit
AAACCGTTACTATTACTGAGTA295680.20384662609635296No Hit
AAACCGTTACCATTACTGAGTCA293120.2020817202427049No Hit
AAACCGTTACCATTACTGGGTA290540.20030302606207517No Hit
TGAGGTAGTAGTTTGTACAGTCA276340.190513313905121No Hit
TGAGGTAGTAGATTGTATA272700.18800383839446513No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA267290.1842740959459354No Hit
TGAGGTAGTAGTTTGTGCTGTCA265590.18310208815249723No Hit
TATTGCACTTGTCCCGGCCTGTTA264270.18219205857170995No Hit
TGAGGTAGTAGATTGTATAGTCA263520.181674996309899No Hit
TCCTGTACTGAGCTGCCCCGA256460.17680771688538516No Hit
TGAGGTAGTAGTTTGTGCTA248320.17119586780386353No Hit
TAGCAGCACGTAAATATTGGCA246690.17007211915486103No Hit
TAGCTTATCAGACTGATGTTGAA241360.1663975300142578No Hit
TCGTACCGTGAGTAATAATGCGA232880.16055127937404856No Hit
AGACCGTTACCATTACTGAGTA225020.1551324668702697No Hit
TACAGTATAGATGATGTACTA222640.15349165595945624No Hit
TCCTGTACTGAGCTGCCCCGAGTA222240.15321588941982375No Hit
TAAAGTGCTTATAGTGCAGGTAGA220390.15194046917402337No Hit
AAACCGTTACCATTACTGAA211030.14548753214662258No Hit
AGCAGCATTGTACAGGGCTATGAA210260.14495668155783No Hit
GAACCGTTACCATTACTGAGTA202200.13939998578423488No Hit
CAAAGTGCTGTTCGTGCAGGTAGA201320.13879329939704335No Hit
AAACCGTTACCATTACCGAGTA200600.13829691962570484No Hit
TAGCTTATCAGACTGATGTTGA191590.1320852783204825No Hit
AATTGCACGGTATCCATCTGTA189870.1308994822000627No Hit
TATTGCACTTGTCCCGGCCTGCA184000.12685260823095557No Hit
TAGCAGCACGTAAATATTGGA182880.12608046191998454No Hit
TGAGGTAGTAGGTTGTGTGGTTA176180.12146137238113998No Hit
TATTGCACTTGTCCCGGCCTGA175050.12068233190667811No Hit
TGAGGTAGTAGTTTGTACAGCA174800.12050997781940781No Hit
AAACCGTTACCATTACTGAAA171750.11840725795470988No Hit
TTCAAGTAATCCAGGATAGGCTA161430.11129248123219108No Hit
TGAGGGGCAGAGAGCGAGACTA155050.10689400492505251No Hit
ACCACAGGGTAGAACCACGGAA146580.10105464844833408No Hit
TGAGGTAGTAGTTTGTACAGA145880.10057205700397717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)