FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007984470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11049881
Sequences flagged as poor quality0
Sequence length0-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA376663034.08751641759762No Hit
AAACCGTTACCATTACTGAGTTA4238103.835425919971446No Hit
TGAGGTAGTAGATTGTATAGTA2807772.540995690360828No Hit
AAACCGTTACCATTACTGAGCA2768962.505873140172279No Hit
AAACCGTTACCATTACTGAGA2667772.414297493339521No Hit
TGAGGTAGTAGTTTGTACAGTTA2658422.4058358637527406No Hit
TGAGGTAGTAGTTTGTGCTGTTA2556812.313880122328919No Hit
TGAGGTAGTAGATTGTATAGTTA2288172.070764381987462No Hit
TCCTGTACTGAGCTGCCCCGAGA2113571.9127536305594601No Hit
TGAGGTAGTAGTTTGTACAGTA1737751.5726413705269768No Hit
TATTGCACTTGTCCCGGCCTGTA1598661.446766711786308No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA1029190.9314036956597089No Hit
AAACCGTTACCATTACTGAGGA884700.8006421064625039No Hit
TGAGGTAGTAGGTTGTATAGTA863380.7813477810304021No Hit
AAACCGTTACCATTACTGAGTTTA814790.7373744567927926No Hit
AAACCGTTACCATTACTGA716320.6482603749307345No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA689990.624432064019513No Hit
CATTGCACTTGTCTCGGTCTGAA683910.618929742320302No Hit
TCCTGTACTGAGCTGCCCCGAA639960.5791555583268273No Hit
TGAGGTAGTAGTTTGTGCTGTA617680.5589924452580077No Hit
TGAGGTAGTAGGTTGTATAGTTA574490.5199060514769345No Hit
TCCTGTACTGAGCTGCCCCGAGAA569280.5151910685734987No Hit
TATTGCACTTGTCCCGGCCTGTAA462380.4184479452765147No Hit
AGCAGCATTGTACAGGGCTATGA403970.3655876475049822No Hit
TAGCAGCACGTAAATATTGGCGA379380.3433340141853111No Hit
TGAGGTAGTAGGTTGTGTGGTA373840.3383203855317537No Hit
TGAGGTAGTAGTTTGTGCTGA347630.3146006730751218No Hit
TCCTGTACTGAGCTGCCCCGAGTA328440.2972339702119869No Hit
CATTGCACTTGTCTCGGTCTGA314170.2843198039870294No Hit
AAACCGTTACCATTACTGAGTCA307210.2780210936208272No Hit
AAACCGTTACCATTACTGAGAA306390.277279004181131No Hit
TACAGTACTGTGATAACTGAAGA296950.26873592575340854No Hit
TGAGGTAGTAGTTTGTACAGTCA296720.268527778715445No Hit
TCCTGTACTGAGCTGCCCCGA296400.26823818283653916No Hit
TAGCTTATCAGACTGATGTTGAA282000.2552063682857761No Hit
TGAGGTAGTAGTTTGTGCTGTCA278260.25182171645106405No Hit
TGAGGTAGTAGATTGTATAGCA269650.24402977733425363No Hit
TATTGCACTTGTCCCGGCCTGTTA250480.22668117421355038No Hit
TGAGGTAGTAGATTGTATAGTCA241130.2182195446267702No Hit
TACAGTATAGATGATGTACTA230910.20897057624421475No Hit
AAACCGTTACCATTACTGGGTA226840.20528727865938104No Hit
CAACGGAATCCCAAAAGCAGCTA224630.20328725712068754No Hit
TGAGGTAGTAGATTGTATAGA224080.2027895142038181No Hit
TAAAGTGCTTATAGTGCAGGTAGA216000.19547721826144554No Hit
AATTGCACGGTATCCATCTGTAA213370.1930971021316881No Hit
TAGCAGCACGTAAATATTGGCA212530.19233691294956026No Hit
TATTGCACTTGTCCCGGCCTGCA211600.19149527492649016No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA207430.18772147862949837No Hit
TAGCTTATCAGACTGATGTTGA207380.18767622927341931No Hit
TGAGGTAGTAGGTTGTGTGGTTA207280.18758573056126127No Hit
AAACCGTTACTATTACTGAGTA201560.18240920422581927No Hit
AAACCGTTACCATTACTGAA196440.17777567016332574No Hit
TGAGGTAGTAGATTGTATA196280.1776308722238728No Hit
TTCAAGTAATCCAGGATAGGCTA189440.17144076031226038No Hit
TGGAGAGAAAGGCAGTTCCTGAA184520.166988223674083No Hit
TCGTACCGTGAGTAATAATGCGA183840.16637283243140807No Hit
TCAGTGCACTACAGAACTTTGTA182880.16550404479469055No Hit
AGCAGCATTGTACAGGGCTATGAA181040.16383886849098195No Hit
CAAAGTGCTGTTCGTGCAGGTAGA175670.15897908764809324No Hit
TGAGGTAGTAGTTTGTACAGCA173370.15689761726845747No Hit
TATTGCACTTGTCCCGGCCTGA159880.14468934099833292No Hit
AATTGCACGGTATCCATCTGTA156030.14120514058024697No Hit
AGACCGTTACCATTACTGAGTA155220.14047210101176655No Hit
TGAGGTAGTAGTTTGTGCTA144870.13110548430340563No Hit
TAGCAGCACGTAAATATTGGA137620.12454432767194507No Hit
TACAGTACTGTGATAACTGAA133160.12050808510969484No Hit
TACCACAGGGTAGAACCACGGAA132350.11977504554121442No Hit
TGAGGTAGTAGTTTGTACA126660.11462566881941987No Hit
GAACCGTTACCATTACTGAGTA124780.11292429303084803No Hit
AAACCGTTACCATTACCGAGTA122560.11091522162093871No Hit
AGAGGTAGTAGGTTGCATAGTA121970.11038127921920607No Hit
CATTGCACTTGTCTCGGTCTGTA120370.10893329982467684No Hit
ACCACAGGGTAGAACCACGGAA119950.10855320523361293No Hit
TGAGGTAGTAGTTTGTACAGA117890.10668893176315655No Hit
GTACAGTACTGTGATAACTGAA117800.10660748292221428No Hit
CCGCACTGTGGGTACTTGCTA115890.104878957519995No Hit
TCCTGTACTGAGCTGCCCCGAAA114120.10327713031479706No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA113920.10309613289048089No Hit
TACAGTACTGTGATAACTGAAA112070.10142190671555648No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCA111820.10119565993516129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)