FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007984471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9480723
Sequences flagged as poor quality0
Sequence length0-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA276515529.16607731288004No Hit
AAACCGTTACCATTACTGAGTTA3582253.7784565586400953No Hit
AAACCGTTACCATTACTGAGCA2363342.4927845692781023No Hit
TGAGGTAGTAGATTGTATAGTA2249112.372297977696427No Hit
TGAGGTAGTAGATTGTATAGTTA2190642.3106254660114No Hit
TGAGGTAGTAGTTTGTGCTGTTA1985512.0942601107531567No Hit
TGAGGTAGTAGTTTGTACAGTTA1888541.991978881779375No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA1828391.92853435333993No Hit
AAACCGTTACCATTACTGAGA1680601.7726496175449908No Hit
TCCTGTACTGAGCTGCCCCGAGA1429171.507448324352478No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA1283461.3537575140630098No Hit
TATTGCACTTGTCCCGGCCTGTA1275251.3450978369476674No Hit
TGAGGTAGTAGTTTGTACAGTA1082421.141706175784273No Hit
AAACCGTTACCATTACTGAGTTTA782770.8256437826524412No Hit
AAACCGTTACCATTACTGA751100.7922391572878988No Hit
TGAGGTAGTAGGTTGTATAGTA672910.7097665441760086No Hit
AAACCGTTACCATTACTGAGGA591030.6234018228356635No Hit
TCCTGTACTGAGCTGCCCCGAA563370.5942268327004174No Hit
TGAGGTAGTAGGTTGTATAGTTA543770.5735533038988694No Hit
TGAGGTAGTAGTTTGTGCTGTA464860.4903212550350854No Hit
CATTGCACTTGTCTCGGTCTGAA446840.47131426580019264No Hit
TCCTGTACTGAGCTGCCCCGAGAA443090.46735887125908016No Hit
TAGCAGCACGTAAATATTGGCGA405910.42814245284879643No Hit
TCCTGTACTGAGCTGCCCCGAGTA366460.38653170227629263No Hit
TATTGCACTTGTCCCGGCCTGTAA329460.34750514280398237No Hit
TACAGTATAGATGATGTACTA312280.3293841619462988No Hit
AGCAGCATTGTACAGGGCTATGA300120.3165581359143179No Hit
TGAGGTAGTAGGTTGTGTGGTA299400.31579870016242434No Hit
AAACCGTTACCATTACTGAGTCA297510.3138051813137036No Hit
TACAGTACTGTGATAACTGAAGA281970.29741402633533326No Hit
TCCTGTACTGAGCTGCCCCGA276250.2913807311952896No Hit
AAACCGTTACCATTACTGAGAA242930.2562357322326578No Hit
TGAGGTAGTAGTTTGTGCTGA242130.2553919147305538No Hit
TGAGGTAGTAGATTGTATAGTCA241530.2547590516039758No Hit
TGAGGTAGTAGATTGTATAGCA226070.23845227837581587No Hit
TGAGGTAGTAGTTTGTGCTGTCA223060.23527741502414953No Hit
TAGCTTATCAGACTGATGTTGAA222480.23466564733512413No Hit
CATTGCACTTGTCTCGGTCTGA222010.234169904552638No Hit
TGAGGTAGTAGTTTGTACAGTCA220510.232587746736193No Hit
TTCAAGTAATCCAGGATAGGCTA212070.22368547208899575No Hit
TGAGGTAGTAGGTTGTGTGGTTA207920.2193081687968312No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCA201420.21245215159223615No Hit
TCAGTGCACTACAGAACTTTGTA197550.20837018442580804No Hit
TATTGCACTTGTCCCGGCCTGTTA195250.20594420910725902No Hit
TGAGGTAGTAGATTGTATA194720.2053851800121151No Hit
TGGAGAGAAAGGCAGTTCCTGAA192940.20350768606993372No Hit
AGCAGCATTGTACAGGGCTATGAA189960.20036446587459628No Hit
TAAAGTGCTTATAGTGCAGGTAGA183890.19396200057738214No Hit
TGAGGTAGTAGATTGTATAGA183720.19378268935818502No Hit
AATTGCACGGTATCCATCTGTAA178600.1883822573447194No Hit
TATTGCACTTGTCCCGGCCTGCA177050.18674736093439287No Hit
AAACCGTTACCATTACTGGGTA176290.18594573430739406No Hit
TAGCAGCACGTAAATATTGGCA173770.18328770917576645No Hit
CAACGGAATCCCAAAAGCAGCTA170860.18021832301186314No Hit
TCGTACCGTGAGTAATAATGCGA166650.17577773340704078No Hit
AAACCGTTACCATTACTGAA153180.16156995621536457No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA150540.15878535845842137No Hit
CAAAGTGCTGTTCGTGCAGGTAGA148570.15670745785949025No Hit
AATTGCACGGTATCCATCTGTA148550.15668636242193765No Hit
AAACCGTTACCATTACTGAGTTTAGTA148280.15640157401497753No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTA145090.15303685172533782No Hit
AAACCGTTACTATTACTGAGTA134710.14208831963553836No Hit
TAGCTTATCAGACTGATGTTGA128620.1356647589007716No Hit
TACAGTACTGTGATAACTGAA126920.13387164670880058No Hit
ACCACAGGGTAGAACCACGGAA125760.13264811133074977No Hit
AGACCGTTACCATTACTGAGTA123180.12992679988646436No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAA119170.12569716465716801No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA118930.12544401940653682No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTA117380.12380912299621032No Hit
TGAGGTAGTAGTTTGTACAGCA115590.1219210813352526No Hit
TGAGGTAGTAGTTTGTACA111960.11809225941945567No Hit
TACCACAGGGTAGAACCACGGAA109040.11501232553677604No Hit
GAACCGTTACCATTACTGAGTA108680.11463260766082925No Hit
TACAGTACTGTGATAACTGAAA102700.1083250718326018No Hit
TATTGCACTTGTCCCGGCCTGA100020.10549828320055338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)