FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007984472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10872647
Sequences flagged as poor quality0
Sequence length0-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA355562732.70249645739442No Hit
AAACCGTTACCATTACTGAGTTA8112397.461283347100296No Hit
AAACCGTTACCATTACTGAGCA3934913.619091100814733No Hit
AAACCGTTACCATTACTGAGA3117192.867001936143057No Hit
TGAGGTAGTAGTTTGTGCTGTTA2016591.8547369375645142No Hit
TGAGGTAGTAGTTTGTACAGTTA1900781.748221937123499No Hit
TGAGGTAGTAGATTGTATAGTA1878741.7279508844534361No Hit
TGAGGTAGTAGATTGTATAGTTA1607431.4784164334591199No Hit
TCCTGTACTGAGCTGCCCCGAGA1511531.3902134411243188No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA1319101.213228020738648No Hit
TGAGGTAGTAGTTTGTACAGTA1221391.1233603003941912No Hit
TATTGCACTTGTCCCGGCCTGTA977280.8988427565063043No Hit
AAACCGTTACCATTACTGAGGA969080.8913008948051013No Hit
AAACCGTTACCATTACTGAGTCA952760.8762907505412436No Hit
AAACCGTTACCATTACTGAGTTTA779760.7171758634304968No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA738560.6792826070781107No Hit
AAACCGTTACCATTACTGA737480.6782892887077084No Hit
TGAGGTAGTAGGTTGTATAGTA652940.6005345340467689No Hit
TCCTGTACTGAGCTGCCCCGAA606890.5581805424198909No Hit
TGAGGTAGTAGTTTGTGCTGTA524590.48248600363830446No Hit
AAACCGTTACCATTACTGAGAA457350.4206427376884396No Hit
TGAGGTAGTAGGTTGTATAGTTA451790.41552898755933126No Hit
TGAGGTAGTAGGTTGTGTGGTA437870.40272621745192316No Hit
TAGCAGCACGTAAATATTGGCGA405000.37249438890088127No Hit
CATTGCACTTGTCTCGGTCTGAA370790.341030109779155No Hit
TCCTGTACTGAGCTGCCCCGAGAA364660.3353921082878898No Hit
AGCAGCATTGTACAGGGCTATGA348710.32072226753981803No Hit
TACAGTATAGATGATGTACTA331290.30470041012092086No Hit
TGAGGTAGTAGTTTGTGCTGA330770.3042221457203568No Hit
TCCTGTACTGAGCTGCCCCGA316490.2910882694894813No Hit
TGAGGTAGTAGTTTGTGCTGTCA308120.28339005211886303No Hit
TGAGGTAGTAGTTTGTACAGTCA297210.2733556970993356No Hit
TACAGTACTGTGATAACTGAAGA265830.2444942800037562No Hit
TATTGCACTTGTCCCGGCCTGTAA265470.24416317388028874No Hit
TGAGGTAGTAGGTTGTGTGGTTA260380.23948170119015175No Hit
TGAGGTAGTAGATTGTATAGTCA247080.2272491694064932No Hit
TGAGGTAGTAGATTGTATAGCA245260.22557524400451884No Hit
TAGCTTATCAGACTGATGTTGAA243400.22386452903327037No Hit
TTCAAGTAATCCAGGATAGGCTA239910.22065463911409983No Hit
AAACCGTTACCATTACTGAAA224230.20623312795862866No Hit
TGGAGAGAAAGGCAGTTCCTGAA208650.19190359072634292No Hit
TCCTGTACTGAGCTGCCCCGAGTA202420.1861736153118923No Hit
AAACCGTTACCATTACTGGGTA202210.18598047007320298No Hit
TGAGGTAGTAGATTGTATA201630.18544702131872762No Hit
CAACGGAATCCCAAAAGCAGCTA194840.17920199193443878No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCA194500.1788892805956084No Hit
TGAGGTAGTAGATTGTATAGA192020.1766083273006104No Hit
CATTGCACTTGTCTCGGTCTGA188710.17356399044317358No Hit
AAACCGTTACTATTACTGAGTA179160.16478048077896762No Hit
TAGCTTATCAGACTGATGTTGACA171850.1580571869941147No Hit
TGAGGTAGTAGTTTGTACAGCA165210.15195011849460394No Hit
TAGCTTATCAGACTGATGTTGA162130.14911732166049352No Hit
ACCACAGGGTAGAACCACGGAA161790.14880461032166317No Hit
AAACCGTTACCATTA161440.14848270159051424No Hit
TATTGCACTTGTCCCGGCCTGCA160620.14772851542039395No Hit
AAACCGTTACCATTACTGAGTAA159970.14713068491968884No Hit
AGACCGTTACCATTACTGAGTA159770.146946737073318No Hit
TACAGTACTGTGATAACTGAA157510.14486812640932792No Hit
AAACCGTTACCATTACTGAA157190.14457380985513463No Hit
AGCAGCATTGTACAGGGCTATGAA154550.14214569828304No Hit
AAACCGTTACCATTACTGAGGTA151090.13896340054082507No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA149680.13766656822391088No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA147880.13601103760657363No Hit
GTACAGTACTGTGATAACTGAA147690.13583628715252136No Hit
TATTGCACTTGTCCCGGCCTGTTA140810.12950848123736566No Hit
AATTGCACGGTATCCATCTGTAA140300.1290394142291201No Hit
TCCTGTACTGAGCTGCCCCGAAA140210.12895663769825325No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTA136760.12578353734835684No Hit
AATTGCACGGTATCCATCTGTA135620.12473503462404326No Hit
TACCACAGGGTAGAACCACGGAA135610.12472583723172471No Hit
TCGTACCGTGAGTAATAATGCGA135520.12464306070085786No Hit
TAAAGTGCTTATAGTGCAGGTAGA129370.11898666442495558No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTA128950.1186003739475769No Hit
TGAGGTAGTAGTTTGTACA128190.11790137213136782No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAA127880.11761625296949309No Hit
GAACCGTTACCATTACTGAGTA127730.11747829208471497No Hit
AAACCGTTACCATTACTGAGTGA127200.11699083029183234No Hit
TGAGGTAGTAGTTTGTGCTA126760.11658614502981657No Hit
TAGCAGCACGTAAATATTGGCA125850.1157491823288294No Hit
TCAGTGCACTACAGAACTTTGTA120130.11048827392262435No Hit
TGAGGTAGTAGGTTGTATAGA117780.1083268867277674No Hit
CAAAGTGCTGTTCGTGCAGGTAGA113690.10456515326948443No Hit
AAACCGTTACCATTACCGAGTA109240.10047231368773402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)