FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007984474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17337148
Sequences flagged as poor quality0
Sequence length0-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA649550837.46583924876225No Hit
AAACCGTTACCATTACTGAGTTA8774615.061161155225761No Hit
AAACCGTTACCATTACTGAGCA5150512.9707942736602355No Hit
AAACCGTTACCATTACTGAGA4491802.590852889990903No Hit
TGAGGTAGTAGATTGTATAGTA3192521.841433204584745No Hit
TGAGGTAGTAGTTTGTACAGTTA3105591.7912923163602226No Hit
TGAGGTAGTAGTTTGTGCTGTTA3029261.7472654671921817No Hit
TCCTGTACTGAGCTGCCCCGAGA2821001.6271419036164425No Hit
TGAGGTAGTAGATTGTATAGTTA2612571.5069202846973446No Hit
TGAGGTAGTAGTTTGTACAGTA2045001.179548100991005No Hit
TATTGCACTTGTCCCGGCCTGTA1991241.1485395406441705No Hit
AAACCGTTACCATTACTGAGGA1795821.0358220394726976No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA1379350.7956037521280894No Hit
AAACCGTTACCATTACTGAGTTTA1312850.757246808990729No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA1180800.6810808790465421No Hit
CATTGCACTTGTCTCGGTCTGAA1079090.62241494391119No Hit
AAACCGTTACCATTACTGA948830.5472814790529561No Hit
TGAGGTAGTAGGTTGTATAGTA933060.5381854039660964No Hit
TCCTGTACTGAGCTGCCCCGAGAA921400.5314599610039668No Hit
TCCTGTACTGAGCTGCCCCGAA862710.497607795699731No Hit
TGAGGTAGTAGTTTGTGCTGTA767350.44260451603689366No Hit
TATTGCACTTGTCCCGGCCTGTAA752270.4339064302848427No Hit
TAGCAGCACGTAAATATTGGCGA750220.4327239982031647No Hit
AGCAGCATTGTACAGGGCTATGA719930.4152528432012001No Hit
TGAGGTAGTAGGTTGTATAGTTA643260.371029883346442No Hit
AAACCGTTACCATTACTGAGAA614010.3541585963273775No Hit
AAACCGTTACCATTACTGAGTCA610120.35191485935287625No Hit
TGAGGTAGTAGGTTGTGTGGTA563790.3251918943069529No Hit
TACAGTACTGTGATAACTGAAGA525150.30290449155766563No Hit
TGAGGTAGTAGTTTGTGCTGA524760.30267954106407813No Hit
TCCTGTACTGAGCTGCCCCGAGTA494250.2850814909118847No Hit
CAACGGAATCCCAAAAGCAGCTA489020.28206484711326224No Hit
TGGAGAGAAAGGCAGTTCCTGAA483990.27916356254212055No Hit
CATTGCACTTGTCTCGGTCTGA470340.2712902952665571No Hit
TAGCTTATCAGACTGATGTTGAA437030.2520772159296327No Hit
TAAAGTGCTTATAGTGCAGGTAGA408220.23545971921102596No Hit
AGCAGCATTGTACAGGGCTATGAA372840.21505267186967547No Hit
TTCAAGTAATCCAGGATAGGCTA372100.21462584272799656No Hit
AATTGCACGGTATCCATCTGTAA371600.21433744465929458No Hit
TCCTGTACTGAGCTGCCCCGA355360.20497027538785503No Hit
CAAAGTGCTGTTCGTGCAGGTAGA355150.2048491481990002No Hit
TGAGGTAGTAGGTTGTGTGGTTA336760.19424186723214223No Hit
AAACCGTTACCATTACTGGGTA335380.19344588856252481No Hit
TGAGGTAGTAGTTTGTACAGTCA324560.18720495435581447No Hit
TACAGTATAGATGATGTACTA320480.1848516261152065No Hit
TATTGCACTTGTCCCGGCCTGTTA320150.1846612833898632No Hit
TGAGGTAGTAGTTTGTGCTGTCA310410.17904328901154906No Hit
AAACCGTTACTATTACTGAGTA310200.17892216182269424No Hit
TGAGGTAGTAGATTGTATAGCA296750.1711642537746116No Hit
TCGTACCGTGAGTAATAATGCGA295440.17040865083461249No Hit
TAGCAGCACGTAAATATTGGCA289290.1668613545895784No Hit
ACCACAGGGTAGAACCACGGAA276570.15952450772180063No Hit
TGAGGTAGTAGATTGTATAGA272820.15736152220653593No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA263300.15187042297845066No Hit
TGAGGTAGTAGATTGTATAGTCA262100.15117826761356598No Hit
TAGCTTATCAGACTGATGTTGA251020.1447873664111306No Hit
TATTGCACTTGTCCCGGCCTGCA247360.14267629254823228No Hit
AAACCGTTACCATTACTGAA243400.14039217984411276No Hit
AGACCGTTACCATTACTGAGTA235770.1359912253157209No Hit
AATTGCACGGTATCCATCTGTA233700.1347972573112948No Hit
TACCACAGGGTAGAACCACGGAA232950.13436466020824187No Hit
TGAGGTAGTAGATTGTATA230540.13297458151709843No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA229800.13254775237541955No Hit
GTACAGTACTGTGATAACTGAA224190.12931192604458358No Hit
AAACCGTTACCATTACCGAGTA220680.12728737160229583No Hit
TACAGTACTGTGATAACTGAAA213760.12329594233146074No Hit
GAACCGTTACCATTACTGAGTA212650.12265569861894239No Hit
TCAGTGCACTACAGAACTTTGTA210670.12151364226688265No Hit
AAACCGTTACCATTACTGAAA210300.12130022769604319No Hit
TATTGCACTTGTCCCGGCCTGA199800.1152438682533021No Hit
TGAGGTAGTAGTTTGTACAGCA198000.11420563520597506No Hit
TGAGGGGCAGAGAGCGAGACTA196910.11357692741620479No Hit
TACAGTACTGTGATAACTGAA196840.11353655168658651No Hit
TGAGGTAGTAGTTTGTGCTA196560.11337504876811341No Hit
TCCTGTACTGAGCTGCCCCGAAA191490.11045069235147556No Hit
TGGAGAGAAAGGCAGTTCCTGA186960.10783780584903585No Hit
TTTGGCAATGGTAGAACTCACACTA185260.10685725241544917No Hit
TAGCAGCACGTAAATATTGGA184600.1064765669647626No Hit
TTTGGCAATGGTAGAACTCACAA175800.10140076095560815No Hit
AAACCGTTACCATTACTGAGGTA174290.10052979878812825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)