FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007984475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990502
Sequences flagged as poor quality0
Sequence length0-50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA46201623.211029177564253No Hit
TGAGGTAGTAGATTGTATAGTA1131205.682988512445604No Hit
TGAGGTAGTAGATTGTATAGTTA887964.4609852188041No Hit
TGAGGTAGTAGTTTGTGCTGTTA642543.22802991406188No Hit
TGAGGTAGTAGTTTGTACAGTTA635153.1909036012021086No Hit
TGAGGTAGTAGTTTGTACAGTA443742.2292868834093107No Hit
AAACCGTTACCATTACTGAGCA408132.050387289236584No Hit
AAACCGTTACCATTACTGAGTTA352391.770357427422831No Hit
TGAGGTAGTAGGTTGTATAGTA262771.320119246300682No Hit
AAACCGTTACCATTACTGAGA253231.2721916380892861No Hit
TATTGCACTTGTCCCGGCCTGTA231211.161566278255435No Hit
TCCTGTACTGAGCTGCCCCGAGA227691.1438822970285887No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA225121.1309709811896698No Hit
TGAGGTAGTAGGTTGTATAGTTA174020.8742518219022136No Hit
AAACCGTTACCATTACTGAGGA172100.8646060139602973No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA160350.8055756788990919No Hit
TGAGGTAGTAGGTTGTGTGGTA128460.645364837613828No Hit
TGAGGTAGTAGTTTGTGCTGTA128180.6439581572889653No Hit
CAACGGAATCCCAAAAGCAGCTA99220.4984672208317299No Hit
TCCTGTACTGAGCTGCCCCGAGAA74720.37538269240623723No Hit
TGAGGTAGTAGATTGTATAGTCA74260.37307171758681984No Hit
CATTGCACTTGTCTCGGTCTGAA72410.36377757972611935No Hit
TGAGGTAGTAGATTGTATAGCA71260.3580001426775758No Hit
TGAGGTAGTAGTTTGTGCTGA70750.35543797494300433No Hit
TGAGGTAGTAGATTGTATAGA70680.3550863048617886No Hit
AAACCGTTACCATTACTGA69200.34765099457322823No Hit
TGAGGTAGTAGTTTGTGCTGTCA62720.3150963927692612No Hit
TGAGGTAGTAGGTTGTGTGGTTA62670.31484519985410714No Hit
AAACCGTTACCATTACTGAGTTTA62350.3132375651971211No Hit
TGAGGTAGTAGATTGTATA62010.31152945337407345No Hit
TATTGCACTTGTCCCGGCCTGTAA58030.2915344973278098No Hit
TGAGGTAGTAGTTTGTACAGTCA56510.28389823270712616No Hit
AGCAGCATTGTACAGGGCTATGA49580.24908289466677253No Hit
AAACCGTTACCATTACTGAGAA49150.24692263559644756No Hit
TCCTGTACTGAGCTGCCCCGAA46680.23451370558783663No Hit
CATTGCACTTGTCTCGGTCTGA39090.1963826210674493No Hit
TACAGTACTGTGATAACTGAAGA38340.19261472734013832No Hit
TGAGGTAGTAGATTGTATAGGTA37590.1888468336128273No Hit
TATTGCACTTGTCCCGGCCTGTTA36450.1831196351473146No Hit
TACAGTATAGATGATGTACTA35550.1785981626745414No Hit
TAGCAGCACGTAAATATTGGCGA34970.1756843248587542No Hit
TGAGGTAGTAGTTTGTGCTGGTA34530.17347382720539845No Hit
TCCTGTACTGAGCTGCCCCGAGTA30470.15307696249488822No Hit
TGAGGTAGTAGATTGTATAGGA30080.1511176577566865No Hit
TGAGGTAGTAGTTTGTACAGCA29880.15011288609607024No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA29500.14820381994089935No Hit
TGAGGTAGTAGTTTGTGCTA29240.14689761678209817No Hit
TGAGGGGCAGAGAGCGAGACTA28980.14559141362329706No Hit
AAACCGTTACCATTACTGGGTA28980.14559141362329706No Hit
AAACCGTTACTATTACTGAGTA28280.1420747128111401No Hit
TGAGGTAGTAGTTTGTACAGGTA27720.13926135216141455No Hit
AGCAGCATTGTACAGGGCTATGAA26650.13388582377711752No Hit
TAAAGTGCTTATAGTGCAGGTAGA26300.13212747337103908No Hit
TAGCTTATCAGACTGATGTTGAA25460.12790743239645075No Hit
TTCAAGTAATCCAGGATAGGCTA25270.12695289931886528No Hit
TGGAGAGAAAGGCAGTTCCTGAA24210.12162760951759909No Hit
TGAGGTAGTAGGTTGTATAGA24180.12147689376850662No Hit
TGAGGTAGTAGATTGTAGAGTA24150.12132617801941419No Hit
AAACCGTTACCATTACTGAGTCA24100.12107498510426012No Hit
TCAGTGCACTACAGAACTTTGTA23260.11685494412967182No Hit
TATTGCACTTGTCCCGGCCTGCA22970.11539802522177822No Hit
TGAGGTAGTAGTTTGTACAGA21890.10997225825445038No Hit
CATTGCACTTGTCTCGGTCTGTA21760.10931915667504982No Hit
TGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGGGCCT21340.10720913618775564No Hit
TGAGGTAGTAGATTA21170.10635508027623183No Hit
TCGTACCGTGAGTAATAATGCGA20790.10444601412106093No Hit
CCGCACTGTGGGTACTTGCTA20190.10143169913921211No Hit
AGAGGTAGTAGGTTGCATAGTA20110.10102979047496562No Hit
TCCTGTACTGAGCTGCCCCGA19950.1002259731464726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)