FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007984476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11690970
Sequences flagged as poor quality0
Sequence length0-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA331052528.316940339424356No Hit
AAACCGTTACCATTACTGAGTTA8854537.5738197942514605No Hit
AAACCGTTACCATTACTGAGCA3297632.8206641536159958No Hit
TGAGGTAGTAGTTTGTGCTGTTA2825902.4171647006193666No Hit
TGAGGTAGTAGATTGTATAGTTA2756842.3580934687198756No Hit
TGAGGTAGTAGTTTGTACAGTTA2713532.321047783032546No Hit
TGAGGTAGTAGATTGTATAGTA2421282.071068525537231No Hit
AAACCGTTACCATTACTGAGA2238401.9146401025748931No Hit
TCCTGTACTGAGCTGCCCCGAGA1870691.6001153026652195No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA1667711.4264941232421262No Hit
TGAGGTAGTAGTTTGTACAGTA1362711.165609012767974No Hit
TATTGCACTTGTCCCGGCCTGTA1351181.155746700231033No Hit
AAACCGTTACCATTACTGAGTTTA1169901.0006868548974124No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA1095860.9373559251285394No Hit
AAACCGTTACCATTACTGA909610.7780449355357169No Hit
AAACCGTTACCATTACTGAGTCA881380.7538980939990437No Hit
AAACCGTTACCATTACTGAGGA793770.6789599152166159No Hit
TGAGGTAGTAGGTTGTATAGTA705430.603397322891086No Hit
TGAGGTAGTAGGTTGTATAGTTA670860.5738274925006223No Hit
TCCTGTACTGAGCTGCCCCGAGAA645940.552511895933357No Hit
CATTGCACTTGTCTCGGTCTGAA640300.5476876597921301No Hit
TCCTGTACTGAGCTGCCCCGAA638760.5463704038244902No Hit
TAGCAGCACGTAAATATTGGCGA604330.5169203239765391No Hit
TGAGGTAGTAGTTTGTGCTGTA574950.49178981726922577No Hit
TCCTGTACTGAGCTGCCCCGAGTA467170.39959900675478593No Hit
TATTGCACTTGTCCCGGCCTGTAA432570.37000351553378374No Hit
TACAGTACTGTGATAACTGAAGA424460.36306653767822517No Hit
TGAGGTAGTAGGTTGTGTGGTA400460.3425378732474722No Hit
AGCAGCATTGTACAGGGCTATGA393130.33626807698591304No Hit
TGAGGTAGTAGTTTGTGCTGTCA354340.3030886230997086No Hit
TGAGGTAGTAGTTTGTACAGTCA350460.2997698223500702No Hit
AAACCGTTACCATTACTGAGAA343910.29416720768251053No Hit
TCCTGTACTGAGCTGCCCCGA342440.2929098269861269No Hit
TAGCTTATCAGACTGATGTTGAA339980.29080563888197475No Hit
TACAGTATAGATGATGTACTA337890.2890179343544633No Hit
TGAGGTAGTAGATTGTATAGTCA331150.28325280109349354No Hit
TTCAAGTAATCCAGGATAGGCTA308640.26399862457948314No Hit
TGAGGTAGTAGGTTGTGTGGTTA300370.25692478896105286No Hit
AAACCGTTACCATTACTGAGTTTAGTA278010.23779891659973465No Hit
CATTGCACTTGTCTCGGTCTGA269690.23068231293040695No Hit
TGAGGTAGTAGTTTGTGCTGA269020.23010922104838177No Hit
TGAGGTAGTAGATTGTATAGCA265020.2266877769765896No Hit
CAACGGAATCCCAAAAGCAGCTA241680.20672365081768235No Hit
TATTGCACTTGTCCCGGCCTGTTA240110.20538073401950396No Hit
TGGAGAGAAAGGCAGTTCCTGAA236480.20227577352435253No Hit
TGAGGTAGTAGATTGTATA228110.19511640180412745No Hit
AATTGCACGGTATCCATCTGTAA223540.19120740195210492No Hit
TAAAGTGCTTATAGTGCAGGTAGA222240.19009543262877246No Hit
AGCAGCATTGTACAGGGCTATGAA209560.17924945492119132No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACCA208420.17827434336073056No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA206240.17640965634160383No Hit
TAGCAGCACGTAAATATTGGCA197790.16918185573994288No Hit
TATTGCACTTGTCCCGGCCTGCA197310.16877128245132783No Hit
TACAGTACTGTGATAACTGAA186660.15966168761018118No Hit
AAACCGTTACCATTACTGGGTA184490.15780555420123393No Hit
TGAGGTAGTAGATTGTATAGA180100.15405051933244204No Hit
AATTGCACGGTATCCATCTGTA179160.1532464799755709No Hit
TAGCTTATCAGACTGATGTTGA176990.15139034656662365No Hit
TCGTACCGTGAGTAATAATGCGA172730.147746508630165No Hit
AAACCGTTACCATTA170380.14573641023798709No Hit
CAAAGTGCTGTTCGTGCAGGTAGA169420.144915263660757No Hit
ACCACAGGGTAGAACCACGGAA168190.1438631696086809No Hit
AAACCGTTACTATTACTGAGTA164140.14039895748599132No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA163150.13955215007822275No Hit
TCAGTGCACTACAGAACTTTGTA162970.1393981850949921No Hit
TGAGGTAGTAGTTTGTACAGCA160600.13737097948245527No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTA156000.13343631879989426No Hit
TGAGGTAGTAGTTTGTACA155600.13309417439271506No Hit
AAACCGTTACCATTACTGAA149260.12767118553892448No Hit
TACAGTACTGTGATAACTGAAA145910.12480572612879855No Hit
TACCACAGGGTAGAACCACGGAA144570.12365954236474817No Hit
GTACAGTACTGTGATAACTGAA144370.12348847016115858No Hit
AAACCGTTACCATTACTGAGTAA142650.12201724921028793No Hit
AAACCGTTACCATTACTGAGGTA139660.1194597197666233No Hit
TGGAAGACTAGTGATTTTGTTGTTA139430.11926298673249525No Hit
TAGCTTATCAGACTGATGTTGACA138400.11838196488400878No Hit
TCCTGTACTGAGCTGCCCCGAAA135990.116320544830754No Hit
AGACCGTTACCATTACTGAGTA135440.11585009627088257No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTA131730.11267670689429535No Hit
TAGCAGCACGTAAATATTGGCAA129660.1109061095871429No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACAA129540.11080346626498913No Hit
TGTAAACATCCTTGACTGGAAGCTA127100.10871638538119592No Hit
AAACCGTTACCATTACTGAGTGA122440.10473040303755805No Hit
AAACCGTTACCATTACTGAAA118530.10138594145738121No Hit
TTTGGCAATGGTAGAACTCACACTA117610.10059900932086902No Hit
GAACCGTTACCATTACTGAGTA117390.10041082989692045No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)