FastQCFastQC Report
Fri 5 May 2023
EGAF00007984477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12881901
Sequences flagged as poor quality0
Sequence length0-50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA424139732.92524139100277No Hit
AAACCGTTACCATTACTGAGTTA6247934.85016147849607No Hit
AAACCGTTACCATTACTGAGCA3749422.9106107864048947No Hit
AAACCGTTACCATTACTGAGA2853752.215317444218831No Hit
TGAGGTAGTAGATTGTATAGTA2786562.163158993381489No Hit
TGAGGTAGTAGTTTGTACAGTTA2618672.03282885033816No Hit
TGAGGTAGTAGTTTGTGCTGTTA2616202.0309114314727306No Hit
TGAGGTAGTAGATTGTATAGTTA2474441.920865561690002No Hit
TCCTGTACTGAGCTGCCCCGAGA1954141.5169655472433767No Hit
TATTGCACTTGTCCCGGCCTGTA1744491.3542178285642779No Hit
TGAGGTAGTAGTTTGTACAGTA1651291.2818682584193124No Hit
AAACCGTTACCATTACTGAGGA1096070.850860443656569No Hit
TGAGGTAGTAGGTTGTATAGTA1001670.7775793339818401No Hit
AAACCGTTACCATTACTGA928900.7210892243310983No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA871750.6767246542261115No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA844390.6554855529475035No Hit
TGAGGTAGTAGGTTGTATAGTTA727050.5643965125954624No Hit
AAACCGTTACCATTACTGAGTTTA715850.5557021436510031No Hit
TGAGGTAGTAGTTTGTGCTGTA678410.5266381103223818No Hit
TCCTGTACTGAGCTGCCCCGAA668180.518696735831148No Hit
TAGCAGCACGTAAATATTGGCGA637850.4951520742163753No Hit
AGCAGCATTGTACAGGGCTATGA599380.4652884694580404No Hit
CATTGCACTTGTCTCGGTCTGAA595130.46198926695679465No Hit
TGAGGTAGTAGGTTGTGTGGTA589310.4574713002374417No Hit
TCCTGTACTGAGCTGCCCCGAGAA548220.42557383417245637No Hit
AAACCGTTACCATTACTGAGTCA534260.414736924309541No Hit
TATTGCACTTGTCCCGGCCTGTAA452650.35138447345620805No Hit
TCCTGTACTGAGCTGCCCCGAGTA369440.28678996989652383No Hit
TGAGGTAGTAGTTTGTGCTGA369010.2864561682316919No Hit
AAACCGTTACCATTACTGAGAA363650.2822952916654149No Hit
TCCTGTACTGAGCTGCCCCGA331430.25728345529126484No Hit
TACAGTACTGTGATAACTGAAGA326640.25356506000162554No Hit
TGAGGTAGTAGGTTGTGTGGTTA323020.2507549157535056No Hit
TGAGGTAGTAGTTTGTACAGTCA313230.2431551057565184No Hit
TAGCTTATCAGACTGATGTTGAA308750.23967735817873467No Hit
TGAGGTAGTAGTTTGTGCTGTCA305420.23709233598364093No Hit
CATTGCACTTGTCTCGGTCTGA303530.23562516122426339No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA290350.22539375205569429No Hit
TGAGGTAGTAGATTGTATAGCA285700.2217840363778607No Hit
TGAGGTAGTAGATTGTATAGTCA280170.2174911917115339No Hit
TACAGTATAGATGATGTACTA279070.21663728047591735No Hit
TATTGCACTTGTCCCGGCCTGTTA269090.20888997672005089No Hit
AGCAGCATTGTACAGGGCTATGAA267550.2076945009901877No Hit
TTCAAGTAATCCAGGATAGGCTA266450.20684058975457115No Hit
AAACCGTTACCATTACTGGGTA261510.20300575202371143No Hit
TGGAGAGAAAGGCAGTTCCTGAA258910.2009874163758905No Hit
AATTGCACGGTATCCATCTGTAA251300.19507990319130694No Hit
TGAGGTAGTAGATTGTATA245240.19037562856600126No Hit
CAACGGAATCCCAAAAGCAGCTA244120.1895061916715553No Hit
TGAGGTAGTAGATTGTATAGA243460.1889938449301854No Hit
TATTGCACTTGTCCCGGCCTGCA243210.188799774194818No Hit
AATTGCACGGTATCCATCTGTA236090.18327263965155452No Hit
TAAAGTGCTTATAGTGCAGGTAGA235530.18283792120433157No Hit
TAGCAGCACGTAAATATTGGCA234080.18171231093920068No Hit
TAGCTTATCAGACTGATGTTGA219420.17033200301725654No Hit
CAAAGTGCTGTTCGTGCAGGTAGA211270.1640052970442794No Hit
TCGTACCGTGAGTAATAATGCGA204050.158400534206869No Hit
AAACCGTTACTATTACTGAGTA196270.15236105292223562No Hit
AAACCGTTACCATTA181440.14084877690024167No Hit
AGACCGTTACCATTACTGAGTA180370.1400181541528692No Hit
TGAGGTAGTAGTTTGTACAGCA179810.13958343570564624No Hit
ACCACAGGGTAGAACCACGGAA178050.13821717772865977No Hit
TCAGTGCACTACAGAACTTTGTA168770.131013272031822No Hit
GAACCGTTACCATTACTGAGTA156640.12159695995179592No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA155230.12050240100432383No Hit
TGAGGTAGTAGTTTGTACA154930.12026951612188294No Hit
TGAGGTAGTAGTTTGTGCTA152820.1186315591153821No Hit
AAACCGTTACCATTACTGAA152330.11825118047406202No Hit
TACCACAGGGTAGAACCACGGAA151600.11768449392678922No Hit
TTTGGCAATGGTAGAACTCACACTA148580.11534011944355108No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG144770.11238248143655195TruSeq Adapter, Index 12 (100% over 49bp)
TATTGCACTTGTCCCGGCCTGA141860.11012349807687546No Hit
GTACAGTACTGTGATAACTGAA139490.10828370750559253No Hit
TCGGGCTGGGGCGA139380.10819831638203088No Hit
CGCGACCTCAGATCAGACGTA138920.10784122622895488No Hit
AGAGGTAGTAGGTTGCATAGTA136920.1062886603460157No Hit
TAGCAGCACGTAAATATTGGA136890.10626537185777161No Hit
AAACCGTTACCATTACCGAGTA135980.10555895438103428No Hit
TACAGTACTGTGATAACTGAA131850.10235290583276489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)