FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007984478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12825176
Sequences flagged as poor quality0
Sequence length0-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA431885133.674789336224315No Hit
TGAGGTAGTAGTTTGTGCTGTTA5032233.9237122359958256No Hit
TGAGGTAGTAGATTGTATAGTA4499483.508318326391778No Hit
AAACCGTTACCATTACTGAGCA3888623.0320207691496788No Hit
TGAGGTAGTAGATTGTATAGTTA3513062.7391904797251905No Hit
TGAGGTAGTAGTTTGTACAGTTA2913292.271539977307134No Hit
TCCTGTACTGAGCTGCCCCGAGA2276731.77520370870544No Hit
TGAGGTAGTAGTTTGTACAGTA2222171.7326623821770553No Hit
AAACCGTTACCATTACTGAGTTA2143441.6712753103739084No Hit
TATTGCACTTGTCCCGGCCTGTA1877261.463730400268971No Hit
AAACCGTTACCATTACTGAGA1574961.228022133965257No Hit
TGAGGTAGTAGGTTGTATAGTA1294841.0096079773096291No Hit
TGAGGTAGTAGGTTGTATAGTTA911320.7105711453784338No Hit
AAACCGTTACCATTACTGAGGA897640.6999046250905251No Hit
TGAGGTAGTAGTTTGTGCTGTA823060.6417533763279349No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA727950.5675945499695287No Hit
TCCTGTACTGAGCTGCCCCGAA717080.559119032752455No Hit
CATTGCACTTGTCTCGGTCTGAA706590.550939807765601No Hit
AAACCGTTACCATTACTGA705740.5502770488295833No Hit
TGAGGTAGTAGTTTGTGCTGTCA665380.5188076951146713No Hit
TCCTGTACTGAGCTGCCCCGAGAA518850.4045558517091695No Hit
TGAGGTAGTAGATTGTATAGCA476950.3718857347454725No Hit
TGAGGTAGTAGGTTGTGTGGTA450470.35123884459753224No Hit
TATTGCACTTGTCCCGGCCTGTAA448080.34937532241272945No Hit
TGAGGTAGTAGATTGTATAGTCA442990.3454065659605763No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA442370.344923141795481No Hit
AAACCGTTACCATTACTGAGAA418430.3262567312916408No Hit
TGAGGTAGTAGTTTGTACAGTCA402330.3137032973270698No Hit
CATTGCACTTGTCTCGGTCTGA391800.30549288368440325No Hit
TCCTGTACTGAGCTGCCCCGAGTA388040.302561150038019No Hit
TGAGGTAGTAGATTGTATAGA376080.293235741950052No Hit
TCAGTGCACTACAGAACTTTGTA362190.2824054812191271No Hit
TGAGGTAGTAGATTGTATA356700.278124838208848No Hit
TGAGGTAGTAGTTTGTGCTGA355480.27717358420656374No Hit
AGCAGCATTGTACAGGGCTATGA351110.27376622355903735No Hit
TCCTGTACTGAGCTGCCCCGA335520.26161044495607705No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA296330.23105335942368355No Hit
TATTGCACTTGTCCCGGCCTGCA286460.22335755860192485No Hit
AAACCGTTACCATTACTGAGTTTA283660.22117435269504293No Hit
TTCAAGTAATCCAGGATAGGCTA276760.21579430956736967No Hit
TGAGGTAGTAGGTTGTGTGGTTA259570.20239098473190542No Hit
TATTGCACTTGTCCCGGCCTGTTA254940.19878089782159716No Hit
TGAGGTAGTAGTTTGTACAGCA251160.19583356984730657No Hit
AAACCGTTACCATTACTGGGTA218260.17018090044144424No Hit
AAACCGTTACTATTACTGAGTA218060.17002495716238122No Hit
AGACCGTTACCATTACTGAGTA216660.1689333542089403No Hit
TACAGTACTGTGATAACTGAAGA215230.1678183597636399No Hit
TACAGTATAGATGATGTACTA215180.16777937394387415No Hit
TGGAGAGAAAGGCAGTTCCTGAA214620.16734273276249775No Hit
AAACCGTTACCATTACTGAGTCA214180.16699965754855917No Hit
TGAGGTAGTAGTTTGTGCTA212680.16583008295558674No Hit
A201040.15675418411412054No Hit
AGCAGCATTGTACAGGGCTATGAA195660.15255930990732602No Hit
GAACCGTTACCATTACTGAGTA194830.1519121452992146No Hit
TACAGTACTGTGATAACTGAA192570.15014998624580278No Hit
CAACGGAATCCCAAAAGCAGCTA186150.14514420698788072No Hit
AATTGCACGGTATCCATCTGTAA185090.14431770760884685No Hit
TGAGGTAGTAGATTA182690.14244638826009093No Hit
TGAGGTAGTAGTTTGTGCTGGTA177600.13847763180793776No Hit
CCGCACTGTGGGTACTTGCTA174770.1362710344091964No Hit
TGAGGTAGTAGTTTGTACA174440.13601372799874248No Hit
TCGTACCGTGAGTAATAATGCGA168370.13128084947918064No Hit
TATTGCACTTGTCCCGGCCTGA167660.130727250838507No Hit
TAGCAGCACGTAAATATTGGCGA161760.1261269241061487No Hit
TGAGGTAGTAGGTTGTATAGA161130.1256357027771003No Hit
TAGCTTATCAGACTGATGTTGAA156160.12176051229238491No Hit
TCGGGCTGGGGCGA152380.11881318431809434No Hit
TAGCTTATCAGACTGATGTTGA152170.1186494438750782No Hit
AAACCGTTACCATTACCGAGTA150210.11712119974026088No Hit
TGAGGTAGTAGTTTGTGCTGTAA146790.1144545696682837No Hit
TCCTGTACTGAGCTGCCCCGAAA143050.11153843034980573No Hit
AGAGGTAGTAGGTTGCATAGTA140480.10953455921384625No Hit
TGAGGTAGTAGGTTGTATAGCA136860.10671198586280609No Hit
CATTGCACTTGTCTCGGTCTGTA134420.10480947785823758No Hit
AAACCGTTACCATTACTAAGTA134400.10479388353033128No Hit
TGAGGTAGTAGTTTGTACAGA133830.10434944518500175No Hit
AAGCCGTTACCATTACTGAGTA128500.10019355679797298No Hit
TGAGGGGCAGAGAGCGAGACTA128400.10011558515844149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)