FastQCFastQC Report
Wed 19 Apr 2023
EGAF00007984479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16237584
Sequences flagged as poor quality0
Sequence length1-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA687316342.32872944644967No Hit
AAACCGTTACCATTACTGAGTTA6861924.225948885006538No Hit
AAACCGTTACCATTACTGAGA6590034.058504023751317No Hit
AAACCGTTACCATTACTGAGCA6352093.9119674454032074No Hit
AAACCGTTACCATTACTGA4201622.5875893852188847No Hit
TGAGGTAGTAGATTGTATAGTA2245681.383013630599232No Hit
AAACCGTTACCATTA1509000.9293254464457273No Hit
AAACCGTTACCATTACTGAGGA1496950.9219043916878275No Hit
TCCTGTACTGAGCTGCCCCGAGA1263740.7782808082778817No Hit
TGAGGTAGTAGTTTGTGCTGTTA1250830.7703301180766794No Hit
TGAGGTAGTAGTTTGTACAGTA1131090.6965876204243193No Hit
TATTGCACTTGTCCCGGCCTGTA1108310.6825584397284719No Hit
TGAGGTAGTAGATTGTATAGTTA1080680.6655423614744657No Hit
TGAGGTAGTAGTTTGTACAGTTA1031880.6354886293428874No Hit
AAACCGTTACCATTACTGAGTCA730880.4501162241870466No Hit
TCCTGTACTGAGCTGCCCCGAA701060.43175142311811904No Hit
AAACCGTTACCATTACTGAGAA684820.42174993521203646No Hit
TCGGGCTGGGGCGA682470.42030267557045436No Hit
TAGCAGCACGTAAATATTGGCGA609370.3752836628897501No Hit
TGAGGTAGTAGGTTGTGTGGTA510340.3142955257383118No Hit
TGAGGTAGTAGGTTGTATAGTA499780.3077920951786916No Hit
AAACCGTTACCATTACTAA488040.30056195552244713No Hit
CATTGCACTTGTCTCGGTCTGAA484870.2986096946442278No Hit
TGAGGTAGTAGATTGTATA476260.2933071816595375No Hit
AAACCGTTACCATTACTGAA458250.2822156301085186No Hit
TGAGGTAGTAGTTTGTGCTGA456330.28103318818858763No Hit
TCGGGCTGGGGCA424160.261221127478078No Hit
TCCTGTACTGAGCTGCCCCGA404640.24919963462544675No Hit
TGAGGTAGTAGATTA378960.23338447394637035No Hit
TGAGGTAGTAGTTTGTGCTGTA374430.23059465004153326No Hit
TGAGGTAGTAGTTTGTACA368070.226677811181762No Hit
AAACCGTTACCATTACTGAAA359670.22150462778206412No Hit
AAACCGTTACCATTACTGAGTTTA338890.20870715741947818No Hit
CGCGACCTCA318300.19602669953855206No Hit
AGACCGTTACCATTACTGAGTA296790.18277965490432566No Hit
TGAGGTAGTAGATTGTATAGA291370.17944171990118726No Hit
AAACCGTTACCATTACTGGGTA278000.1712077363233348No Hit
CATTGCACTTGTCTCGGTCTGA272100.16757419084021366No Hit
TGAGGTAGTAGATTGTATAGCA263800.16246259295717885No Hit
AAACCGTTACTATTACTGAGTA261470.16102765041892933No Hit
GAACCGTTACCATTACTGAGTA254450.1567043471491818No Hit
CGGGGCTGGGCGA240250.14795920378302585No Hit
TACAGTATAGATGATGTACTA238300.14675828620809597No Hit
TCCTGTACTGAGCTGCCCCGAGAA234260.14427023133490793No Hit
TAGCTTATCAGACTGATGTTGA233320.1436913274782751No Hit
CGGGGCTGGGCA230930.14221943363002773No Hit
TGAGGTAGTAGGTTGTATAGTTA230510.14196077446004282No Hit
TAGCTTATCAGACTGATGTTGAA221650.13650429768369482No Hit
AGCAGCATTGTACAGGGCTATGA215920.1329754475789009No Hit
TATTGCACTTGTCCCGGCCTGTAA214940.13237190951560282No Hit
AAACCGTTACCATTACTA207960.12807324045252053No Hit
GGCTGGGCGCGA204590.12599780854097506No Hit
TGAGGTAGTAGTTTGTGCTA195690.12051669755796184No Hit
AAGCCGTTACCATTACTGAGTA193600.11922956025970366No Hit
CAACGGAATCCCAAAAGCAGCTA190490.11731425069148219No Hit
AAACCGTTACCATTACCGAGTA187940.1157438200165739No Hit
TAGCAGCACGTAAATATTGGCA177920.10957295124693427No Hit
TGAGGTAGTAGTTTGTACAA177710.10944362166194183No Hit
AAACCGTTACCATTACTAAGTA177350.10922191380195478No Hit
TGAGGTAGTAGGTTGTGTGGTTA177340.10921575525028847No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGTTG175150.10786703243536723TruSeq Adapter, Index 13 (97% over 45bp)
TACAGTACTGTGATAACTGAAGA166610.10260762931234105No Hit
TACAGTATAGATGATGA165680.10203488400737448No Hit
TGAGGTAGTAGTTTGTGCTGTCA163930.10095713746577077No Hit
AAACCGTTACCACTACTGAGTA163540.1007169539507848No Hit
TTCAAGTAATCCAGGATAGGCTA163400.1006307342274565No Hit
ACCACAGGGTAGAACCACGGAA162840.10028585533414332No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)