FastQCFastQC Report
Mon 22 May 2023
EGAF00007984480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13886676
Sequences flagged as poor quality0
Sequence length0-50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA499643235.98004302829561No Hit
AAACCGTTACCATTACTGAGTTA9953067.167345158769455No Hit
AAACCGTTACCATTACTGAGA3941452.83829622006015No Hit
AAACCGTTACCATTACTGAGCA3516952.5326075152901963No Hit
TGAGGTAGTAGATTGTATAGTA3473802.5015345644990927No Hit
TGAGGTAGTAGTTTGTGCTGTTA2662171.9170678425852234No Hit
TGAGGTAGTAGTTTGTACAGTTA2377521.7120871834267608No Hit
TGAGGTAGTAGATTGTATAGTTA2255521.624233185825031No Hit
TGAGGTAGTAGTTTGTACAGTA1954181.4072338117487582No Hit
TCCTGTACTGAGCTGCCCCGAGA1748371.2590269982535778No Hit
TATTGCACTTGTCCCGGCCTGTA1647101.1861009790967976No Hit
AAACCGTTACCATTACTGAGGA1212340.8730238971514854No Hit
TAGCAGCACGTAAATATTGGCGA900520.648477720658277No Hit
AAACCGTTACCATTACTGA878840.6328656332156091No Hit
AAACCGTTACCATTACTGAGTTTA844580.6081945024136806No Hit
TGAGGTAGTAGGTTGTATAGTA793650.5715190589886305No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA791610.570050024930372No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA769860.5543875294562932No Hit
AAACCGTTACCATTACTGAGTCA679930.4896276113880672No Hit
TCCTGTACTGAGCTGCCCCGAA671330.4834346246718797No Hit
TGAGGTAGTAGTTTGTGCTGTA641760.46214083197447686No Hit
CATTGCACTTGTCTCGGTCTGAA625740.45060459392874147No Hit
TGAGGTAGTAGGTTGTGTGGTA550000.39606310394222494No Hit
TGAGGTAGTAGTTTGTGCTGA468860.33763299438973016No Hit
TGAGGTAGTAGGTTGTATAGTTA446990.3218840851475184No Hit
AGCAGCATTGTACAGGGCTATGA431620.3108159216791693No Hit
TCCTGTACTGAGCTGCCCCGAGAA429240.3091020486112011No Hit
TATTGCACTTGTCCCGGCCTGTAA401320.2889964452256249No Hit
TACAGTACTGTGATAACTGAAGA387850.27929649975271265No Hit
AAACCGTTACCATTACTGAGAA373670.2690852728183476No Hit
TGAGGTAGTAGATTGTATAGCA336950.24264265976969576No Hit
TGAGGTAGTAGATTGTATAGA331300.23857401152010746No Hit
TAGCTTATCAGACTGATGTTGAA319250.22989662897010052No Hit
CAACGGAATCCCAAAAGCAGCTA306350.22060714889581928No Hit
TTCAAGTAATCCAGGATAGGCTA294830.21231142715506576No Hit
CATTGCACTTGTCTCGGTCTGA292470.2106119563817864No Hit
AAACCGTTACTATTACTGAGTA292260.21046073228755388No Hit
TCCTGTACTGAGCTGCCCCGA287820.2072634228666385No Hit
TGAGGTAGTAGTTTGTGCTGTCA283970.20449098113904293No Hit
AAACCGTTACCATTACTGGGTA283940.2044693776970097No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA282250.2032523837958054No Hit
TGAGGTAGTAGGTTGTGTGGTTA270030.19445258174094363No Hit
TGAGGTAGTAGTTTGTACAGTCA268050.1930267545667516No Hit
TATTGCACTTGTCCCGGCCTGTTA267460.19260188687343177No Hit
TGAGGTAGTAGATTGTATA257380.18534313035027244No Hit
TCCTGTACTGAGCTGCCCCGAGTA256480.1846950270892761No Hit
TGAGGTAGTAGATTGTATAGTCA254670.18339161941993892No Hit
TAGCTTATCAGACTGATGTTGA249140.17940938493848346No Hit
TAGCAGCACGTAAATATTGGCA236420.17024952551640146No Hit
AATTGCACGGTATCCATCTGTAA232490.16741947461005066No Hit
AGACCGTTACCATTACTGAGTA220150.15853325878705601No Hit
AAACCGTTACCATTA215300.15504070232502004No Hit
TATTGCACTTGTCCCGGCCTGCA214660.15457982889497818No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA211710.15245549042837897No Hit
AATTGCACGGTATCCATCTGTA211290.152153042239914No Hit
TCGTACCGTGAGTAATAATGCGA209530.1508856403072989No Hit
TAAAGTGCTTATAGTGCAGGTAGA202380.14573681995604995No Hit
TGAGGTAGTAGTTTGTACAGCA193350.13923418390405307No Hit
TGGAGAGAAAGGCAGTTCCTGAA193010.13898934489434334No Hit
GTACAGTACTGTGATAACTGAA190860.13744109821529643No Hit
TACAGTATAGATGATGTACTA186630.13439501288861352No Hit
AAACCGTTACCATTACTGAGGTA185760.13376851306965037No Hit
AAACCGTTACCATTACCGAGTA183970.13247950769500202No Hit
AGCAGCATTGTACAGGGCTATGAA176030.12676179670354518No Hit
GAACCGTTACCATTACTGAGTA175570.1264305439257026No Hit
TGAGGTAGTAGTTTGTACA172030.12388133776578354No Hit
AAACCGTTACCATTACTGAA169560.12210265437171573No Hit
ACCACAGGGTAGAACCACGGAA167480.12060481572407969No Hit
TAGCAGCACGTAAATATTGGCAA165980.11952464362241907No Hit
TGAGGTAGTAGTTTGTGCTA158260.11396535787253911No Hit
AAACCGTTACCATTACTGAAA158090.11384293836768425No Hit
TATTGCACTTGTCCCGGCCTGA157520.1134324729690532No Hit
TGAGGTAGTAGGTTGTATAGA151970.10943583619290895No Hit
TACAGTACTGTGATAACTGAA150880.1086509111323689No Hit
TAGCAGCACGTAAATATTGGA150320.10824764688108227No Hit
CAAAGTGCTGTTCGTGCAGGTAGA149620.107743566566974No Hit
TGAGGTAGTAGTTTGTACAGA146690.10563363039506359No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCGA143210.10312763111921097No Hit
TACCACAGGGTAGAACCACGGAA141280.10173780968174098No Hit
TCAGTGCACTACAGAACTTTGTA139780.10065763758008035No Hit
TGAGGGGCAGAGAGCGAGACTA139020.10011035038190565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)