FastQCFastQC Report
Sat 22 Apr 2023
EGAF00007984481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007984481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14988803
Sequences flagged as poor quality0
Sequence length0-50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGTA638420842.59318105655268No Hit
AAACCGTTACCATTACTGAGTTA5903153.9383731976462695No Hit
AAACCGTTACCATTACTGAGCA4766103.179773594996212No Hit
AAACCGTTACCATTACTGAGA4391512.929860376442335No Hit
TGAGGTAGTAGATTGTATAGTA3532802.356959391620532No Hit
TGAGGTAGTAGATTGTATAGTTA2900131.9348643117132167No Hit
TGAGGTAGTAGTTTGTACAGTTA2832861.8899841434969824No Hit
TGAGGTAGTAGTTTGTGCTGTTA2177571.4527977984632927No Hit
TGAGGTAGTAGTTTGTACAGTA2069331.3805838931901366No Hit
TCCTGTACTGAGCTGCCCCGAGA1993801.3301929446934488No Hit
TATTGCACTTGTCCCGGCCTGTA1835661.224687521745399No Hit
AAACCGTTACCATTACTGAGGA1697991.1328389598555668No Hit
CATTGCACTTGTCTCGGTCTGAA996160.6646027704814053No Hit
AAACCGTTACCATTACTGA969130.6465693091036022No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTA921650.6148923299612384No Hit
AAACCGTTACCATTACTGAGTTTA913390.6093815496807851No Hit
TATTGCACTTGTCCCGGCCTGTAA736040.49105989317492527No Hit
TGAGGTAGTAGGTTGTATAGTA699190.4664748746114016No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA645000.43032122044702303No Hit
AAACCGTTACCATTACTGAGAA640880.42757250195362495No Hit
TCCTGTACTGAGCTGCCCCGAGAA588890.3928866100915463No Hit
TCCTGTACTGAGCTGCCCCGAA557610.37201769881157287No Hit
TGAGGTAGTAGGTTGTATAGTTA496770.33142739950615135No Hit
TGAGGTAGTAGTTTGTGCTGTA489360.32648370920613207No Hit
AGCAGCATTGTACAGGGCTATGA470450.31386762505318133No Hit
CAACGGAATCCCAAAAGCAGCTA392600.26192885449225No Hit
TGAGGTAGTAGTTTGTGCTGA391360.26110157028549913No Hit
TAGCAGCACGTAAATATTGGCGA380630.2539428932383727No Hit
CATTGCACTTGTCTCGGTCTGA378460.25249514587655864No Hit
AAACCGTTACCATTACTGAGTCA371380.24777161992188437No Hit
AAACCGTTACCATTACTGGGTA334250.22299979524715882No Hit
TCCTGTACTGAGCTGCCCCGAGTA332870.22207910798480707No Hit
AAACCGTTACTATTACTGAGTA331630.2212518237780562No Hit
TGAGGTAGTAGATTGTATAGCA319800.21335926557977977No Hit
TAGCTTATCAGACTGATGTTGAA312330.2083755453987887No Hit
TGAGGTAGTAGGTTGTGTGGTA310670.2072680520252351No Hit
TATTGCACTTGTCCCGGCCTGTTA305360.20372540755922938No Hit
TGAGGTAGTAGTTTGTACAGTCA299890.2000760167439655No Hit
TGAGGTAGTAGATTGTATAGTCA292110.19488547551128665No Hit
TGGAGAGAAAGGCAGTTCCTGAA285830.19069568130290324No Hit
AATTGCACGGTATCCATCTGTAA272530.18182239102081735No Hit
TGAGGTAGTAGATTGTATAGA270040.18016115096048696No Hit
TAAAGTGCTTATAGTGCAGGTAGA266100.17753252211000436No Hit
AAACCGTTACCATTACTGAA259730.17328268308016323No Hit
TACAGTATAGATGATGTACTA253880.16937976968541116No Hit
TGAGGTAGTAGATTGTATA241840.1613471069037334No Hit
TTCAAGTAATCCAGGATAGGCTA235490.15711061116754954No Hit
TCCTGTACTGAGCTGCCCCGA226770.15129293513297892No Hit
TAGCAGCACGTAAATATTGGCA226230.15093266620423257No Hit
AAACCGTTACCATTACTGAAA226150.1508792930296035No Hit
AGCAGCATTGTACAGGGCTATGAA224990.15010538199748172No Hit
TGAGGTAGTAGTTTGTGCTGTCA224870.15002532223553808No Hit
CAAAGTGCTGTTCGTGCAGGTAGA224450.1497451130687354No Hit
TATTGCACTTGTCCCGGCCTGCA222950.14874436604443997No Hit
TACAGTACTGTGATAACTGAAGA222040.1481372461830341No Hit
AGACCGTTACCATTACTGAGTA220080.14682960340462145No Hit
AAACCGTTACCATTACCGAGTA210260.14027804621890086No Hit
CCCCCCACTGCTAAATTTGACTGGCTTA202510.13510751992670797No Hit
GAACCGTTACCATTACTGAGTA200230.13358638444977894No Hit
TCGTACCGTGAGTAATAATGCGA198180.13221869684990856No Hit
TGAGGTAGTAGTTTGTGCTA192350.12832912674881378No Hit
TGAGGTAGTAGTTTGTACAGCA190330.1269814540894293No Hit
TAGCTTATCAGACTGATGTTGA187820.12530687073544167No Hit
TCAGTGCACTACAGAACTTTGTA185300.12362561573462537No Hit
TATTGCACTTGTCCCGGCCTGA182280.1216107783923773No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGA175680.11720749148547752No Hit
AAACCGTTACCATTACTGAGT173190.11554625142514716No Hit
TAGCAGCACGTAAATATTGGA171980.11473898215888222No Hit
CATTGCACTTGTCTCGGTCTGTA163280.10893464941796886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)