FastQCFastQC Report
Wed 8 May 2024
EGAF00007985083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031858
Total Bases304.7 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGCAGAAGACGGCATACGAGATCGGACGAACGCACTTGTGAACTCCGG21590.10625742546969326RNA PCR Primer, Index 22 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)