FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8200932
Sequences flagged as poor quality0
Sequence length150
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACACCTATAGGCCTGAGCAGCAATAGATCGGAAGAGCGTCGACTAGGG384510.46886134405211505No Hit
CGACACCTATAGGCCTGAGCAGCAATAGATCGGAAGAGCGTCGACTACGG203620.24828885302304668No Hit
CGACACCTATAGGCCTGAGCAGCAATAGATCCGAAGAGCGTCGACTAGGG125160.15261680013930126No Hit
CGACACCTATAGGCCTGAGCAGCAATAGATCGGAAGAGCGTCGACTAGTG118730.14477622787263691No Hit
CGACACCTATAGGCCTGAGCAGCAATAGATCGGAAGAGCGTCGAGTAGGG110980.13532608245014102Illumina Single End PCR Primer 1 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)