FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8873553
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGAA988411.113883018448191Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGAA702060.7911825173073289Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGAA646000.7280060196856885No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACACGTCTGAA579630.653210726300953Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGTC273950.30872639178466615No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCACACGTCTG256840.28944437476172175Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGATGCACTCCATGACCATACGTACGATAGATCGGAAGTGCACACGTCTG233340.26296118364312465Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGATGCACTCCATGACCATACGTACGATCGATCGGAAGTGCACACGTCTG216150.24358901107594666Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGGC186750.21045684857012742No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGTC175490.19776745571925924No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCAGACGTCTGAA165910.18697132929729499Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGTTAAGACACCGCCTTGATCCACTGTCAGATCGGAAGTGCACACGTCTG151260.17046159525953133Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGGC150180.16924449541237876No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGTC141060.1589667633697573No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCAGAAGTCTGAA117310.13220183617543052No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCAGAAGTCTGAA111590.1257557147627337No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCAGACGTCTGAA98010.11045181112909339No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)