FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8873553
Sequences flagged as poor quality0
Sequence length150
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGGG670750.7558978911829343No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTACGG334260.37669240269371246No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGTCGAGT259840.29282520767047876No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTAGGG238690.2689903356637414No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGTG212550.2395320115854382No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGAGTAGGG208180.23460726498168208Illumina Single End PCR Primer 1 (95% over 24bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTACGG143440.16164889081070458No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGACTAGGG133180.15008644226275542No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGAGCGTCGAGT121780.13723927720947854No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGTCGAGT99750.11241269421617248No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGACTACGG93700.1055946811835124No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGACGAGT91620.10325063703344084No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)