FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6255448
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCACACGTCTG2849434.555117395268892Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGAACACGGC1063181.6996064870173966No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGGC945321.5111947217849147No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCAAACGTCTG918791.4687836906325495No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGGC776031.2405666228861625No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGAACACGTC762311.2186337413403485No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGAAGTGAACACGGC659291.0539452969635428No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGAA639091.0216534451249535Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCAGAACGGAAGTGCACACGTCTG596780.9540164029818489No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCACACGTCGG500420.7999746780726177No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGCACACGGC467490.7473325651496104No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGTC457310.7310587507081826No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGCACACGTC456680.7300516285963851No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGAA442320.7070956388735067Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGAA411590.6579704603091577No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGAA410500.6562279791950952Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGAAGTGAACACGTC393500.629051668241827No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGGC391590.625998329775901No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACACGTCTGAA376210.6014117613958264Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCACTCCATGACCATACGTACGATAGATCGGAAGTGCACACGTCTG292420.4674645205267472Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGTC280150.4478496184445942No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGTC250800.4009305168870399No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGAACGGAAGTGCAAACGTCTG248630.39746154072418155No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCCGATCGGAAGTGCACACGTCTG227820.36419453890432785Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACAAGTCTGAA221700.3544110669611513Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACACGTCTGAA220050.3517733661921576Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGGACACGTCTG219240.3504784949055607No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACAAGTCTGAA213510.34131847950778266No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGCACACGGC210130.33591518944766224No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCAAACGTCGG209850.3354675796201967No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGAAGTGCACACGGC195480.31249560383205166No Hit
CTCATCCGACCGTCACAGGAGTCGCAAGATCGGAAGAGCACACGTCTGAA158220.2529315246485943Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCCGATCGGAAGTGCACACGTCGG148880.23800053968956342No Hit
AACTCCAACACGATTAGCCGCGAATAAGATCGGAAGAGCACACGTCTGAA148520.23742504133996478Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCACTCCATGACCATACGTACGATCGATCGGAAGTGCACACGTCTG145460.23253330536837652Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCAGAACGGAAGTGCACACGTCGG137930.22049579822260532No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGAAGTGCACACGTC127570.2039342346063783No Hit
TCGTCTGTCTATGATCGGATGTACTGAGATCGGAAGAGCACACGTCTGAA108370.1732409892944518Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCATGGGTCGTACACAAGTGAATCAGATCGGAAGAGCACACGTCTGAA105150.16809347627859747Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTAACGCAGGCATTCGTATGCTGGAGAGATCGGAAGAGCACACGTCTGAA101210.1617949665635459Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAGTCCCGTGTAACCTTGCTAATGAGGACACATCGGAAGTGAACACGGC98230.15703111911409062No Hit
CCTTAGCGTTCTGTGTTATGTTAGCTAGATCGGAAGAGCACACGTCTGAA87210.13941447519026615Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCCGATCGGAAGTGCAAACGTCTG86370.13807164570786937No Hit
CGATGCACTCCATGACCATACGTACGATAGATCGGAAGTGCAAACGTCTG85500.1366808580296727No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGGACACGTCGG82670.13215680155921686No Hit
TTTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGAGCACACGTCT81760.13070206961995368Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGCACACGTC81580.13041432044515436No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCCGAACGGAAGTGCACACGTCTG79630.12729703771816184No Hit
AGAGTCCCGTGTAACCTTGCTAATGAGGACACATCGGATGTGAACACGGC76780.12274100911717274No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGAGAACACGTC75260.12031112719664523No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGAGCACACGTCTG73720.11784927314558447Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCCGATCGGAAGTGCAAACGTCGG73130.11690609529485338No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGGAAACGTCTG73010.11671426251165383No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGAGAACACGGC71270.1139326871552605No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGAACGGAAGTGCAAACGTCGG67610.1080817872676745No Hit
ATGCGCGCTGAACGTCTCCTTCATTCGATGAGATCGGAAGTGAACACGTC67310.10760220530967567No Hit
TCGTCTGTCTATGATCGGATGTACTGAGATCGGAAGAGCACAAGTCTGAA66500.10630733402307876Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCCGAACGGAAGTGCACACGTCGG66240.10589169632614644No Hit
CGATGCACTCCATGACCATACGTACGATAGAACGGAAGTGCACACGTCTG64790.10357371686248532No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCCGATCGGAAGTGGACACGTCGG63890.10213497098848875No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)