FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6255448
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGTCGAGT1649462.636837521469286No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGTCGAGT645971.0326518580283939No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGAGCGTCGAGT635411.0157705731068343No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGACGAGT621480.9935019841904209No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCTACGTGAAG481650.7699688335671562No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGGG406430.6497216506315775No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCGTCCTGTAG403180.6445261794199232Illumina Single End PCR Primer 1 (95% over 22bp)
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGACGAGT385730.6166304955296567No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCTACCTGTAG329460.5266769062743388No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGAGCGACGAGT317910.508213000891383No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGATCGTCGAGT309470.4947207618063486No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGTCGTGT249110.3982288718569797No Hit
GAAACGCATAGGCGCAAAGCCAATACCTCGATCGGAAGAGCTACGTGAAG235520.3765038091596317No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGATCGACGAGT227140.36310748646619717No Hit
GAAACGCATAGGCGCAAAGCCAATACCTCGATCGGAAGAGCGTCCTGTAG212880.34031135739598506No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCGTCCTGAAG204870.32750651911741574No Hit
GAAACGCATAGGCGCAAAGCCAATACCTCGATCGGAAGAGCTACCTGTAG202840.32426134786829014No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGAACGGAAGAGCTACGTGAAG201030.3213678700550304No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTACGG189430.3028240343457415No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCTACCTGAAG182330.29147392800643535No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCTACGTGTAG178350.2851114740303173No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAACATCGTCGAGT158230.25294751071386096No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAACAGCGTCGAGT142860.22837692839905313No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGACGTGT142440.2277055136578547No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAACATCGTCGAGT133870.2140054557243542No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGTG131140.20964125990656468No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTAGGG127740.204205997715911No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCTTCCTGTAG127050.20310295921251365No Hit
GAAACGCATAGGCGCAAAGCCAATACCTCGAACGGAAGAGCTACGTGAAG126800.20270330758084795No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGAGTAGGG126780.20267133545031468Illumina Single End PCR Primer 1 (95% over 24bp)
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGTCGTGT126650.2024635166018485No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTAGGG126400.20206386497018278No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGAGTCGAGT125150.20006560681185426No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGACTAGT119090.19037805126027743No Hit
GAAACGCATAGGCGCAAAGCCAATACCTCGATCGGAAGAGCTACGTGTAG118840.18997839962861174No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGAACGGAAGAGCGTCCTGTAG109100.17440797205891567No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAACAGCGTCGAGT106160.16970806887052695No Hit
GAAACGCATAGGCGCAAAGCCAATACCTCGATCGGAAGAGCTACCTGAAG89380.1428834513531245No Hit
GTCCTCATTAGCAAGGTTACACGGGACTCTAGATCGGAAGAGCGTCGAGT88380.14128484482646167No Hit
ATCGTACGTATGGTCATGGAGTGCATCGAGATCGGAAGAGCTACGTGAAG87000.13907876781966694No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAATAGCTACGTGAAG85880.13728832850980457No Hit
TGCGACTCCTGTGACGGTCGGATGAGAGATCGGAAGAGCGTCGACTAGGG84860.13565774985260848No Hit
GGAGTAAGAACGGGACAGGGCGATGACGCAAGATCGGAAGAGCGTCGAGT84360.13485844658927706No Hit
GAAACGCATAGGCGCAAAGCCAATACCTCGAACGGAAGAGCTACCTGTAG83600.1336435056290133No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGAACGGAAGAGCTACCTGTAG82550.13196496877601732No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCGACGTGAAG81520.1303184040535546No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGACTAGGG79030.12633787380216413No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCGTCGTGTAG78240.12507497464610048Illumina Single End PCR Primer 1 (100% over 22bp)
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTACGG76410.12214952470230749No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGAGCGTCGTGT73060.11679419283798698No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCTTCCTGAAG72780.11634658301052139No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTACGG71090.11364493798046119No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGACGTGT70110.11207830358433161No Hit
GAAACGCATAGGCGCAAAGCCAATACCTCGATCGGAAGAGCGTCCTGAAG69870.11169463801793253No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCGACCTGTAG66960.10704269302534368No Hit
GAAACGCATAGGCGCAAAGCCAATACCTCGAACGGAAGAGCGTCCTGTAG66810.10680290204634424No Hit
CATCGAATGAAGGAGACGTTCAGCGCGCATAGATCGGAAGAGCGTCGAGT66770.10673895778527773No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGTCTAGT65970.10546007256394746No Hit
ATCGTACGTATGGTCATGGAGTGCATCGAGATCGGAAGAGCGTCCTGTAG65910.10536415617234769Illumina Single End PCR Primer 1 (95% over 22bp)
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAACATCGACGAGT65440.10461281110481614No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGAGCGACTAGT63900.10215095705375539No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)