FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5696076
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGAA584101.025442778502253Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACACGTCTGAA345010.6056976767866159Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACAAGTCTGAA344370.6045740962725918No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACAAGTCTGAA338020.5934260708600095Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TCGTCTGTCTATGATCGGATGTACTGAGATCGGAAGAGCACACGTCTGAA254700.44714993269050485Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGTC252890.4439723065492806No Hit
AGAATGAAGCGAGTAATTTGGACCAGATCGGAAGAGCACACGTCTGAACT246240.4322976027707495Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGCATGGGTCGTACACAAGTGAATCAGATCGGAAGAGCACACGTCTGAA221580.38900464108976074Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCTGTCTATGATCGGATGTACTGAGATCGGAAGAGCACAAGTCTGAA176960.3106700121276472Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGTC173000.30371785769712345No Hit
GGCTCAGATACTTGATATCTTTGAAGATCGGAAGAGCACACGTCTGAACT144780.2541749794068759Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGTCTGTCTATGATCGGATGTACTGAGATCCGAAGAGCACAAGTCTGAA144420.25354296536773735No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCACACGTCTG136880.24030578243689166Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGAA135010.23702282062247768Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGGC130360.2288593059502717No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGTC127320.22352229850865754No Hit
TCGTCTGTCTATGATCGGATGTACTGAGATCCGAAGAGCACACGTCTGAA124740.21899286456149814Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACGCATGGGTCGTACACAAGTGAATCAGATCGGAAGAGCACAAGTCTGAA120790.21205826607650602Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGAGTCCCGTGTAACCTTGCTAATGAGGACACATCGGATGTGAACACGTC117490.20626480405106953No Hit
ATGCGCGCTGAACGTCTCCTTCATTCGATGAGATCGGATGTGAACACGTC116050.20373674789451543No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGGC113770.19973399231330483No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGAA109580.19237805113555367No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGAA103670.1820024873263629Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
ACGCATGGGTCGTACACAAGTGAATCAGATCCGAAGAGCACACGTCTGAA103290.18133536139616116Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AACTCCAACACGATTAGCCGCGAATAAGATCGGAAGAGCACACGTCTGAA97740.17159181162610893Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGCATGGGTCGTACACAAGTGAATCAGATCCGAAGAGCACAAGTCTGAA94980.16674637065938025No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACACGTCTGAA89980.15796839789356745Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGTTAAGACACCGCCTTGATCCACTGTCAGATCGGAAGTGCACACGTCTG87950.15440454095064743Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACGATACGAGATTACGATAGACTGTCGCAGATCGGAAGTGCACACGTCTG87020.15277183801620625Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GCTTACGGCCAACTGAGTGATTAATTCCTGAGATCGGATGTGAACACGTC86600.15203448830387797No Hit
TTGCCAGATTGACGGCGACGCTGCATGTAGATCGGAAGTGCACACGTCTG84330.14804928866819894Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGAGTCCCGTGTAACCTTGCTAATGAGGACACATCGGATGTGAACACGGC81610.14327407148359678No Hit
CCGCGTTTGCTATGGTTTATTTCTGCTGTTAGATCGGATGTGAACACGTC70610.12396253139880858No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGCA68800.12078490525758434Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCAGACGTCTGAA62850.11033911766626708Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGAGTCCCGTGTAACCTTGCTAATGAGGCCACATCGGATGTGAACACGGC58780.10319384783489546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)