FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5696076
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGTCGAGT209390.3676039434867091No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTAGGG170850.2999433294078239No Hit
CAGTACATCCGATCATAGACAGACGAAGATCGGAAGAGCGTCGACTAGGG112180.19694259697377636No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTACGG109330.19193915249726304No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGAGCGTCGAGT106620.18718149125819247No Hit
GATTCACTTGTGTACGACCCATGCGTAGATCGGAAGAGCGTCGACTAGGG91180.1600751113573625No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGGG87600.15379008285704052No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTCGACTAGGG86640.15210471208600446No Hit
GTCCTCATTAGCAAGGTTACACGGGACTCTAGATCGGAAGAGCGTCGAGT81240.14262450149892664No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGACTAGTG79110.13888508510069036No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGACGAGT78580.1379546199875142No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGTCGAGT72330.1269821540302482No Hit
CAGTACATCCGATCATAGACAGACGAAGATCGGAAGAGCGTCGACTACGG70290.12340074114179655No Hit
CATCGAATGAAGGAGACGTTCAGCGCGCATAGATCGGAAGAGCGTCGAGT67980.11934531772399104No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCCGAAGAGCGTCGACTACGG65660.1152723383606539No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGATCGTCGACTAGGG63120.11081312819562099No Hit
TCGGTATGGACTGGTTTGTTGCATCGAGATCGGAAGAGCGTCGAGTAGGG58110.10201759948427654Illumina Single End PCR Primer 1 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)