FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9058809
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCAATTTCAGATGTAGGCGCCTTAGAGATCGGAAGTGCACACGTCTG495560.5470476306543167Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGAGCGGCACAGTTAGATAGCACCTCCTACAGATCGGATGTGAACACGTC276520.3052498402383801No Hit
ACCGCAATTTCAGATGTAGGCGCCTTAGAGATCGGAAGTGCAAACGTCTG215610.23801142070662928No Hit
ACCGCAATTTCAGATGTAGGCGCCTTAGAGAACGGAAGTGCACACGTCTG181490.20034642523095472No Hit
CGAGCGGCACAGTTAGATAGCACCTCCTACAGATCGGAAGTGAACACGTC156000.17220806841164218No Hit
CGAGCGGCACAGTTAGATAGCACCTCCTACAGATCGGATGTGCACACGTC142450.15725025221306688No Hit
GATGTGTCACTGATGTTCGTGGCAGTAGATCGGAAGAGCACACGTCTGAA113590.12539175955691303Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGAGCGGCACAGTTAGATAGCACCTCCTACACATCGGATGTGAACACGTC104710.11558914643194265No Hit
CGAGCGGCACAGTTAGATAGCACCTCCTACAGATCGGATGTGAACACGGC93870.10362289347308239No Hit
ACCGCAATTTCAGATGTAGGCGCCTTAGAGAACGGAAGTGCAAACGTCTG92250.10183457891649995No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)