FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3035809
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGCAATCTAGATCGGAAGAGCACACGTCTGAACTCCTGTCACTATACC63840.21028990954305754TruSeq Adapter, Index 10 (97% over 35bp)
AGTGCAATCTAGATCGGAAGAGCACACGTCTCAACTCCTGTCACTATACC63790.2101252087993678Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)