FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7500547
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTCTGTCTATGATCGGATGTACTGAGATCGGAAGAGCACACGTCTGAA286580.3820788003861585Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGTCTGTCTATGATCGGATGTACTGAGATCGGAAGAGCACAAGTCTGAA183520.24467548833438413Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TCGTCTGTCTATGATCGGATGTACTGAGATCCGAAGAGCACAAGTCTGAA145130.1934925546096838No Hit
TCGTCTGTCTATGATCGGATGTACTGAGATCCGAAGAGCACACGTCTGAA130090.17344068372613358Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTTACTACCGCGTATGTTACCTAGAGATCGGAAGAGCACACGTCTGAACT77410.10320580618986856Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)