FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10573875
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGAA852970.8066768332328498Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGAA620870.5871735763851946Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGAA611460.5782742844983509No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACACGTCTGAA537690.5085079973046778Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCAGACGTCTGAA123060.11638117530233713Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)