FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10573875
Sequences flagged as poor quality0
Sequence length150
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGGG566810.5360475700724663No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTACGG314680.29760139967608845No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGTG198760.18797271577354566No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTAGGG189930.17962194559704933No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGAGTAGGG172690.1633176106205152Illumina Single End PCR Primer 1 (95% over 24bp)
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGATCGTCGACTAGGG135960.12858105472213355No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCCGAAGAGCGTCGACTACGG125040.11825371493421286No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)