FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13911750
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGTC2500541.7974302298416807No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCACACGTCTG2029721.458996891117221Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGGC1787711.2850360306934785No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGTC1684301.2107031825615038No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGAACACGGC1540681.1074667097956763No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGTC1231120.8849497726741783No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGAACACGTC1051320.755706507089331No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCAAACGTCTG723270.5198986468273222No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGCACACGGC660040.47444785882437507No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGCACACGTC566890.40749007134257015No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGAAGTGAACACGTC566530.40723129728466945No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGAACACGGC490410.3525149603752224No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGCACACGTC470780.3384045860513594No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCAGAACGGAAGTGCACACGTCTG463020.3328265674699445No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGATGTGCACACGGC397260.2855571728934174No Hit
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGAAGTGAACACGGC317570.2282746599097885No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGAA295360.2123097381709706Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCACTCCATGACCATACGTACGATAGATCGGAAGTGCACACGTCTG247220.1777058960950276Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACAAGTCTGAA205610.14779592790267218Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCAGAACGGAAGTGCAAACGTCTG204250.14681833701726957No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACAAGTCTGAA197750.14214602763850703No Hit
AGGTATTGGCTTTGCGCCTATGCGTTTCCGATCGGAAGTGCACACGTCTG192820.1386022606789225Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGTGCACACGGC192230.13817815875069636No Hit
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCCGAAGAGCACACGTCTGAA179340.12891260984419645Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCACATCGGAAGTGCACACGTC176960.12720182579474187No Hit
TCGTCTGTCTATGATCGGATGTACTGAGATCGGAAGAGCACACGTCTGAA172440.12395277373443313Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCATCCGACCGTCACAGGAGTCGCAAGATCGGAAGAGCACACGTCTGAA160870.11563606304023577Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGAGCACACGTCT147370.1059320358689597Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGAAGAGAACACGTC142660.1025464086114256No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)