FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13911750
Sequences flagged as poor quality0
Sequence length150
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGTCGAGT2473681.7781228098549786No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGTCGAGT1413521.0160619620105307No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGACGAGT989530.7112908153179865No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGAGCGTCGAGT983780.7071576185598505No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGATCGTCGAGT726850.5224720110697791No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGACGAGT691820.4972918576023865No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGAGCGACGAGT516880.37154204179919853No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAAGATCGACGAGT413830.2974679677251244No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAACATCGTCGAGT392950.2824590723668841No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAACATCGTCGAGT352310.2532463564972056No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGTCGTGT328780.23633259654608513No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAACAGCGTCGAGT270220.194238683127572No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCTACGTGAAG263810.18963106726328463No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGATCGTCGTGT243950.17535536506909627No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAACAGCGTCGAGT229970.16530630582061925No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGACTAGT219360.15767965928082378No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGCGACGTGT207410.14908979819217566No Hit
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCGTCCTGTAG201710.14499254227541467Illumina Single End PCR Primer 1 (95% over 22bp)
GAAACGCATAGGCGCAAAGCCAATACCTAGATCGGAAGAGCTACCTGTAG182950.13150753859147843No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAACATCGGAACATCGACGAGT177950.1279134544539688No Hit
GCGGAGGTACTGGAGCTCCGGTTCAGAGAAAGATCGGAAGAGAGTCGAGT161200.11587327259331141No Hit
AGCGCCTGAGAACCTCTCCAAAGTTGAGATCGGAAGAGCGTCGACTAGGG150660.10829694323144104No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)