FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15735637
Sequences flagged as poor quality0
Sequence length150
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCACACGTCTG825370.5245227759130437Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCAAACGTCTG391040.24850598676113334No Hit
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCACACGTCTG281130.1786581629965155No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCACACGTCTG238120.15132530065354202Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
CAACTTTGGAGAGGTTCTCAGGCGCTAGATCGGAAGAGCACACGTCTGAA182700.11610588119184498Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCAAACGTCTG166660.10591245845338196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)