FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20856021
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCACACGTCTG1882150.9024492255737563Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCAAACGTCTG909620.436142637178971No Hit
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCACACGTCTG588330.28209120042600644No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCACACGTCTG512890.2459193918149584Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGAACGGAAGTGCAAACGTCTG361290.17323055054461253No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGATCGGAAGTGCAAACGTCTG316430.1517211744272793No Hit
AGTCGGTTAGTAGATCATGGTTACATGACGAACGGAAGTGCACACGTCTG271450.13015426096857113No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)