FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15097125
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTAAGGACAGTCACCTAACAGAGATCGGAAGAGCACACGTCTGAACT4896113.2430744264222495Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTTTGGTTACGCCATCGGTTGGGATAGATCGGAAGAGCACACGTCTGAA3083172.0422232709870256Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTTGGTTACGCCATCGGTTGGGATAGATCGGAAGAGCACAAGTCTGAA1709201.1321360855129703Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TCTTTGGTTACGCCATCGGTTGGGATAGATCCGAAGAGCACACGTCTGAA1260480.8349139322884324Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
TCTTTGGTTACGCCATCGGTTGGGATAGATCCGAAGAGCACAAGTCTGAA1194840.7914354554261159No Hit
AGCCTAAGGACAGTCACCTAACAGAGATCGGAAGAGCAGACGTCTGAACT454320.30093146873990906Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
TCCTAGGCTGCTCCGCATCTCGACAAAGATCGGAAGAGCACACGTCTGAA340140.22530117489257062Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGCTGTGTTCGTCATTTAGTCCGAGATCGGAAGAGCACACGTCTGAACT339720.22502297622891773Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAAAGGTGTAGGACCCGCTCGTAGACCGAGATCGGAAGTGCACACGTCTG322900.2138817821273918Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGCCTAAGGACAGTCACCTAACAGAGATCGGAAGAGCAGAGGTCTGAACT312080.20671485464947795No Hit
AATCAGAGTTGCAAGTTAAACCGCTAAGATCGGAAGAGCACACGTCTGAA258660.17133063414391814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGCTGCTCAGGCCTATAGGTGTCGAGATCGGAAGAGCACACGTCTGAA222600.14744529173600934Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTTGGTTACGCCATCGGTTGGGATAGATCGGAAGAGCACACGTCTGCA222250.14721345951629863Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGCCTAAGGACAGTCACCTAACAGAGATCGGAAGAGCACACGGCTGAACT218090.14445796799059424Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
CACCTCCACACATCTACGACCAACGTAGATCGGAAGAGCACACGTCTGAA185060.12257963022760955Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTTGGTTACGCCATCGGTTGGGATAGCTCGGAAGAGCACACGTCTGAA171840.11382299610025089Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGCCTAAGGACAGTCACCTAACAGAGATCGGCAGAGCACACGTCTGAACT168900.11187560545468093Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)