FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15097125
Sequences flagged as poor quality0
Sequence length150
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCCAACCGATGGCGTAACCAAAGAAGATCGGAAGAGCGTCGACTAGGG1278810.8470553168235674No Hit
CTGTTAGGTGACTGTCCTTAGGCTAGATCGGAAGAGCGTCGTCTCGGGAA1024610.6786788875365343No Hit
ATCCCAACCGATGGCGTAACCAAAGAAGATCGGAAGAGCGTCGACTACGG770790.5105541618023299No Hit
CTGTTAGGTGACTGTCCTTAGGCTAGATCGGAAGAGCTTCGTCTCGGGAA475820.3151725908078525No Hit
ATCCCAACCGATGGCGTAACCAAAGAAGATCCGAAGAGCGTCGACTAGGG436460.28910140175695703No Hit
CTGTTAGGTGACTGTCCTTAGGCTAGATCGGAAGAGCGTCGTCTAGGGAA426150.28227228694205025Illumina Single End PCR Primer 1 (96% over 26bp)
ATCCCAACCGATGGCGTAACCAAAGAAGATCGGAAGAGCGTCGACTAGTG424770.28135820561861946No Hit
ATCCCAACCGATGGCGTAACCAAAGAAGATCGGAAGAGCGTCGAGTAGGG409860.27148215305894335Illumina Single End PCR Primer 1 (95% over 24bp)
CTGTTAGGTGACTGTCCTTAGGCTAGATCGGAAGAGCGTCGTGTCGGGAA386050.2557109383409093Illumina Single End PCR Primer 1 (96% over 26bp)
ATCCCAACCGATGGCGTAACCAAAGAAGATCCGAAGAGCGTCGACTACGG302290.2002301762752842No Hit
TTGTCGAGATGCGGAGCAGCCTAGGAAGATCGGAAGAGCGTCGACTAGGG237690.15744057229439382No Hit
CTGTTAGGTGACTGTCCTTAGGCTAGATCGGAAGAGCGTCGTCTAGGCAA237530.1573345918510975Illumina Single End PCR Primer 1 (95% over 23bp)
ATCCCAACCGATGGCGTAACCAAAGAAGATCGGAAGATCGTCGACTAGGG224770.14888265149821572No Hit
CTGTTAGGTGACTGTCCTTAGGCTAGATCGGAAGAGCGTCGTGTAGGGAA214260.1419210611291885Illumina Single End PCR Primer 1 (100% over 26bp)
CTGTTAGGTGACTGTCCTTAGGCTAGATCGGAAGAGCTTCGTCTAGGGAA183690.1216721726818848No Hit
ATCCCAACCGATGGCGTAACCAAAGAAGATCGGAAGATCGTCGACTACGG181190.12001622825537975No Hit
ATCCCAACCGATGGCGTAACCAAAGAAGATCGGAAGAGCGTAGACTAGGG167180.11073631568924547No Hit
CTGTTAGGTGACTGTCCTTAGGCTAGATCGGAAGAGCGTCGTCTCGGCAA166380.11020641347276386No Hit
ATCCCAACCGATGGCGTAACCAAAGAAGATCGGAAGAGCGTAGACTACGG162670.10774899194383038No Hit
CTGTTAGGTGACTGTCCTTAGGCTAGATCGGAAGAGCTTCGTCTAGGCAA157110.10406617153928313No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)