FastQCFastQC Report
Fri 4 Aug 2023
EGAF00007985499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007985499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13309894
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGAA1697121.2750815295749163Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACACGTCTGAA937610.7044458806358638Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACAAGTCTGAA915630.6879318497953477Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACAAGTCTGAA892390.6704711547665219No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACACGTCTGCA377850.28388655837529586Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGGTATTGGCTTTGCGCCTATGCGTTTCAGATCGGAAGTGCACACGTCTG281140.21122632531859384Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACAAGTCTGCA250790.18842373951287666No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCCGAAGAGCACACGTCTGCA245940.18477983370866816Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCACAAGTCTGCA235890.17722905982572063Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTAACGCAGGCATTCGTATGCTGGAGAGATCGGAAGAGCACACGTCTGAA169140.12707839746883032Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCTCTGAACCGGAGCTCCAGTACCTCCGCAGATCGGATGTGAACACGTC159730.12000846888788146No Hit
CGATGCAACAAACCAGTCCATACCGAAGATCGGAAGAGCAGACGTCTGAA147700.1109700798518756Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
AGTCGGTTAGTAGATCATGGTTACATGAAGATCGGAAGTGCACACGTCTG146710.11022627227534644Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)